WB result of ADORA1 Recombinant Rabbit mAb
Primary antibody: ADORA1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HT-29 whole cell lysate 25 µg
Lane 2: Raji whole cell lysate 25 µg
Lane 3: SK-BR-3 whole cell lysate 25 µg
Lane 4: U-2 OS whole cell lysate 25 µg
Lane 5: Caco-2 whole cell lysate 25 µg
Lane 6: SH-SY5Y whole cell lysate 25 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 35 kDa
ADORA1 Recombinant Rabbit mAb
ADORA1 Recombinant Rabbit mAb
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ADORA1 |
| Synonyms | Adenosine receptor A1 |
| Location | Cell membrane |
| Accession | P30542 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HT-29, Raji, SK-BR-3, U-2 OS, Caco-2, SH-SY5Y, mouse testis, rat testis |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt |
| IHC-P | 1:200 | Hu, Ms, Rt |
Background
ADORA1 protein (adenosine A1 receptor) is a cell-surface integral membrane protein of 326 amino acids with a molecular weight of approximately 37 kDa, encoded by the human ADORA1 gene. It belongs to the G protein-coupled receptor (GPCR) family and has a typical seven-transmembrane structure. As the subtype with the highest affinity for adenosine among the four adenosine receptor subtypes (A1, A2A, A2B, and A3), ADORA1 is widely expressed in the central nervous system and inhibits adenylyl cyclase activity by coupling to Gi/o family heterotrimeric G proteins, thereby reducing intracellular cAMP levels. This receptor regulates a variety of physiological functions, including heart rate and myocardial contractility, smooth muscle tone, sedation, neurotransmitter release, platelet function, lipolysis, renal function, leukocyte function, and sperm capacitation. ADORA1 is also involved in processes such as signal transduction, apoptosis, phagocytosis, inflammatory responses, and nervous system development, and it co-localizes with adenosine deaminase, which may be required for high-affinity agonist binding. In addition, ADORA1 can form homo- or hetero-oligomers with other adenosine receptors or non-adenosine receptors, expanding its biological roles in developmental, physiological, and pathological contexts.
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Western Blot
WB result of ADORA1 Recombinant Rabbit mAb
Primary antibody: ADORA1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse testis lysate 25 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 35 kDa
WB result of ADORA1 Recombinant Rabbit mAb
Primary antibody: ADORA1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat testis lysate 25 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 35 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human brain. Anti-ADORA1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cerebellum. Anti-ADORA1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-ADORA1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-ADORA1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-ADORA1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse brain. Anti-ADORA1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebellum. Anti-ADORA1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat brain. Anti-ADORA1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
