ADGRE1 (F4/80) Recombinant Rabbit mAb (S-2683-205)

Catalog Number: S0B60024 Application: IHC-P Reactivity: Hu Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100µl

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Product Details

Product Specification

Host	Rabbit
Antigen	ADGRE1 (F4/80)
Synonyms	Adhesion G protein-coupled receptor E1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; EMR1; TM7LN3
Immunogen	Recombinant Protein
Location	Cell membrane
Accession	Q14246
Clone Number	S-2683-205
Antibody Type	Recombinant mAb
Isotype	IgG
Application	IHC-P
Reactivity	Hu
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
IHC-P	1:500	Hu

Background

ADGRE1 (Adhesion G protein-coupled receptor E1, also known as EMR1 or F4/80) is a structurally unique macrophage marker membrane protein that plays an important role in immune surveillance and inflammatory regulation. This protein is characterized by a long extracellular N-terminal region containing six epidermal growth factor (EGF)-like modules, connected via a serine/threonine-rich domain to the classic C-terminal seven-transmembrane G protein-coupled receptor domain—a conformation that endows it with dual potential for both cell adhesion and signal transduction. As the most classic macrophage surface marker F4/80 in mice, as well as a functionally conserved receptor in humans, ADGRE1 is primarily localized to the cell membrane and participates in regulating macrophage biological functions, mediating peripheral immune tolerance, and maintaining tissue homeostasis through activation of the adenylate cyclase signaling pathway. Although its specific endogenous ligands remain incompletely defined, studies have shown that this receptor is associated with various disease states, including obesity, fibrosis, and inflammatory bowel disease, highlighting its potential research value in immune-related pathological processes.

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Immunohistochemistry

IHC shows positive staining in paraffin-embedded human colon. Anti-ADGRE1 (F4/80) antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver. Anti-ADGRE1 (F4/80) antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human spleen. Anti-ADGRE1 (F4/80) antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human stomach. Anti-ADGRE1 (F4/80) antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-ADGRE1 (F4/80) antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.