ACVR1B Recombinant Rabbit mAb

Catalog Number: S0B60757 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl

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Product Details

Product Specification

Host	Rabbit
Antigen	ACVR1B
Synonyms	Activin receptor type-1B; Activin receptor type IB (ACTR-IB); Activin receptor-like kinase 4 (ALK-4); Serine/threonine-protein kinase receptor R2 (SKR2); ACVRLK4; ALK4
Location	Cell membrane
Accession	P36896
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt
Positive Sample	Caco-2, 293T, HeLa, Hep G2, U-87 MG, MCF7, mouse brain, mouse testis, rat brain, rat testis
Purification	Protein A
Concentration	1.2 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Ms, Rt
IHC-P	1:1000	Hu, Ms, Rt

Background

ACVR1B (activin A receptor type 1B, also known as ALK4) is a type I transmembrane serine/threonine kinase receptor encoded by the ACVR1B gene. The human protein is approximately 505 amino acids long with a molecular weight of about 57 kDa, and the gene is located at chromosome 12q13.13. As a key type I receptor in TGF-β superfamily signal transduction, ACVR1B mainly mediates activin A signaling: after the ligand binds the type II receptor (ACVR2A/ACVR2B), ACVR1B is recruited, the type II receptor phosphorylates its GS domain and activates its kinase activity, which in turn phosphorylates SMAD2/3 to initiate the canonical signaling pathway. In tumor biology, ACVR1B is primarily regarded as a tumor suppressor gene, and its inactivating mutations are found in pancreatic cancer and ER-negative breast cancer; knockdown of ACVR1B can promote pancreatic cancer cell growth and tumorigenesis. Mechanistically, loss of ALK4 increases cell-surface stability of TGF-β receptors by altering their glycosylation, thereby paradoxically enhancing TGF-β signaling and promoting epithelial–mesenchymal transition and metastasis. In addition, ACVR1B is involved in the regulation of skeletal muscle atrophy and regeneration; its genetic variants are associated with pituitary tumors, and in systemic sclerosis it is implicated in activin/ACVR1B signaling-mediated collagen production.

Picture

Western Blot

WB result of ACVR1B Recombinant Rabbit mAb
Primary antibody: ADORA1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Caco-2 whole cell lysate 25 µg
Lane 2: 293T whole cell lysate 25 µg
Lane 3: Hep G2 whole cell lysate 25 µg
Lane 4: U-87 MG whole cell lysate 25 µg
Lane 5: MCF7 whole cell lysate 25 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 55 kDa

WB result of ACVR1B Recombinant Rabbit mAb
Primary antibody: ADORA1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 25 µg
Lane 2: mouse testis lysate 25 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 55 kDa

WB result of ACVR1B Recombinant Rabbit mAb
Primary antibody: ADORA1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 25 µg
Lane 2: rat testis lysate 25 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 55 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human colon. Anti-ACVR1B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-ACVR1B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-ACVR1B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-ACVR1B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse stomach. Anti-ACVR1B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse testis. Anti-ACVR1B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat brain. Anti-ACVR1B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat testis. Anti-ACVR1B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.