WB result of β-Actin Recombinant Rabbit mAb
Primary antibody: β-Actin Recombinant Rabbit mAb at 1/20000 dilution
Lane 1: SW480 whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 40 kDa
β-Actin Recombinant Rabbit mAb (S-5701)
β-Actin Recombinant Rabbit mAb (S-5701)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | β-Actin |
| Synonyms | Actin, cytoplasmic 1; ACTB |
| Location | Cytoplasm, Cytoskeleton, Nucleus |
| Accession | P60709 |
| Clone Number | S-5701 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | SW480, A549, Jurkat, MCF7, NIH/3T3, C2C12, mouse brain, mouse testis, mouse lung, C6, rat brain, rat testis, rat lung |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:5000-1:50000 | Hu, Ms, Rt |
| IHC-P | 1:2000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu, Ms, Rt |
Background
β-actin is a fundamental component of the microfilament system within the cytoskeleton and is a highly conserved member of the actin protein family. Present in almost all eukaryotic cells, it exists in dynamic equilibrium between its monomeric (G-actin) and polymeric (F-actin) states. By polymerizing into helical microfilaments, it provides mechanical support and builds a network that governs intracellular transport, cell migration, division, and morphological maintenance. As a representative cytoplasmic actin, beta-actin not only mediates cell-matrix adhesion (e.g., focal adhesions) and cell-cell connections but also plays unconventional roles in transcriptional regulation and signal transduction by interacting with various proteins. Due to its extremely high and consistent expression levels across most tissues and cell types, the gene encoding beta-actin (ACTB) is widely used as a loading control or housekeeping gene reference in molecular biology experiments such as Western blot and qRT-PCR.
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Western Blot
WB result of β-Actin Recombinant Rabbit mAb
Primary antibody: β-Actin Recombinant Rabbit mAb at 1/20000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: C2C12 whole cell lysate 20 µg
Lane 3: mouse brain lysate 20 µg
Lane 4: mouse testis lysate 20 µg
Lane 5: mouse lung lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 40 kDa
WB result of β-Actin Recombinant Rabbit mAb
Primary antibody: β-Actin Recombinant Rabbit mAb at 1/20000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Lane 3: rat testis lysate 20 µg
Lane 4: rat lung lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 40 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-β-Actin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-β-Actin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical cancer. Anti-β-Actin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-β-Actin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-β-Actin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-β-Actin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-β-Actin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-β-Actin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-β-Actin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti- β-Actin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in C6 cells. Anti- β-Actin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
