WB result of β-actin Recombinant Rabbit mAb (HRP Conjugate)
Primary antibody: β-actin Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Predicted MW: 42 kDa
Observed MW: 42 kDa
β-actin Recombinant Rabbit mAb (HRP Conjugate) (S-R015)
β-actin Recombinant Rabbit mAb (HRP Conjugate) (S-R015)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | β-Actin |
| Synonyms | ACTB |
| Location | Cytoplasm, Cytoskeleton |
| Accession | P60709 |
| Clone Number | S-R015 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | HRP |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied. |
Dilution
| application | dilution | species |
| WB | 1:5000-1:10000 | null |
Background
Beta-actin (human gene and protein abbreviation ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actin is often used in Western blotting as a loading control, to normalize total protein amounts and check for eventual protein degradation in the samples. Its transcript is also commonly used as a housekeeping gene standard in qPCR. Its molecular weight is approximately 42 kDa.
Picture
Picture
Western Blot
WB result of β-actin Recombinant Rabbit mAb (HRP Conjugate)
Primary antibody: β-actin Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Predicted MW: 42 kDa
Observed MW: 42 kDa
WB result of β-actin Recombinant Rabbit mAb (HRP Conjugate)
Primary antibody: β-actin Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Lane 1: C6 whole cell lysate 20 µg
Predicted MW: 42 kDa
Observed MW: 42 kDa
