Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized A431 (Human epidermoid carcinoma epithelial cell) cells labelling β-actin (Alexa Fluor® 594 Conjugate) antibody at 1/200 (1 μg) dilution / (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
β-actin Recombinant Rabbit mAb (Alexa Fluor® 594 Conjugate) (S-R156)
β-actin Recombinant Rabbit mAb (Alexa Fluor® 594 Conjugate) (S-R156)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | β-Actin |
| Synonyms | ACTB |
| Location | Cytoplasm, Cytoskeleton, Nucleus |
| Accession | P60709 |
| Clone Number | S-R156 |
| Antibody Type | Recombinant mAb |
| Application | ICC, ICFCM |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Alexa Fluor® 594 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 0.1%BSA, 0.01% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| ICC | 1:200 | null |
| ICFCM | 1:200 | null |
Background
Beta-actin (human gene and protein abbreviation ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two non-muscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actin is often used in Western blotting as a loading control, to normalize total protein amounts and check for eventual protein degradation in the samples. Its transcript is also commonly used as a housekeeping gene standard in qPCR. Its molecular weight is approximately 42 kDa.
Picture
Picture
FC
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling β-actin (Alexa Fluor® 594 Conjugate) antibody at 1/200 (1 μg) dilution / (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized C6 (Rat glial tumor glial cell) cells labelling β-actin (Alexa Fluor® 594 Conjugate) antibody at 1/200 (1 μg) dilution / (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-β-actin (Alexa Fluor® 594 Conjugate) antibody was used at 1/200 dilution (Red) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
ICC shows positive staining in NIH/3T3 cells. Anti-β-actin (Alexa Fluor® 594 Conjugate) antibody was used at 1/200 dilution (Red) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
ICC shows positive staining in HeLa cells. Anti-β-actin (Alexa Fluor® 594 Conjugate) antibody was used at 1/200 dilution (Red) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
