ACSS3 His Tag Protein, Human

Catalog Number: UA085090 Brand: UA BIOSCIENCE

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$470 USD

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Product Details

Product Specification

Species	Human
Accession	Q9H6R3
Amino Acid Sequence	Gly59-Ala686 with His Tag at the N-Terminus
Expression System	E.coli
Molecular Weight	70-100kDa (Reducing)
Purity	＞95% by SDS-PAGE & HPLC
Conjugation	Unconjugated
Tag	His Tag
Physical Appearance	Liquid
Storage Buffer	50mM Tris, 150mM NaCl, pH8.0, 1mM DTT, 10%Glycerol
Stability & Storage	Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
Reference	1. Zhou L, Song Z, Hu J, Liu L, Hou Y, Zhang X, Yang X, Chen K. ACSS3 represses prostate cancer progression through downregulating lipid droplet-associated protein PLIN3. Theranostics. 2021 Jan 1;11(2):841-860. 2. ACSS3 promotes the tumorigenesis of non-small cell lung cancer via suppressing p53-mediated ferroptosis. (2024). 3. Bowman, C.E., et al. (2019). Role of the malonyl-CoA synthetase ACSF3 in mitochondrial metabolism. Advances in Biological Regulation, 71, 34-40.

Background

ACSS3 (Acyl-CoA Synthetase Short-Chain Family Member 3) is also localized in the mitochondrial matrix and is encoded by the ACSS3 gene on chromosome 12q21.31. Functionally, ACSS3 catalyzes the synthesis of acetyl-CoA from short-chain fatty acids. In contrast to ACSS1, which prefers acetate, ACSS3's preferred substrate is propionate, with much lower affinity for acetate and butyrate. This enzyme is involved in the ketone body biosynthetic process.

ACSS3 exhibits a complex, bidirectional role in cancer. In prostate cancer (PCa), ACSS3 is downregulated due to promoter methylation, and its low expression predicts poor prognosis. Restoring ACSS3 expression reduces lipid droplet (LD) deposits by downregulating the LD coat protein PLIN3, thereby increasing ER stress, promoting apoptosis, and inhibiting the progression of castration-resistant prostate cancer (CRPC). Conversely, in glioma, non-small cell lung cancer, and ovarian cancer, ACSS3 is highly expressed and is associated with promoting tumorigenesis and malignant progression. This disparity suggests that ACSS3's function is highly tissue/tumor type-specific.