ACSS2 Recombinant Rabbit mAb (S-4117-9)

Catalog Number: S0B60043 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100µl

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Product Details

Product Specification

Host	Rabbit
Antigen	ACSS2
Synonyms	Acetyl-coenzyme A synthetase, cytoplasmic; Acetate--CoA ligase; Acetyl-CoA synthetase (ACS; AceCS); Acetyl-CoA synthetase 1 (AceCS1); Acyl-CoA synthetase short-chain family member 2; Acyl-activating enzyme; Propionate--CoA ligase; ACAS2
Immunogen	Synthetic Peptide
Location	Cytoplasm, Nucleus
Accession	Q9NR19
Clone Number	S-4117-9
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt
Positive Sample	HepG2, Caco-2, U-87 MG, Neuro-2a, mouse liver, mouse brain, rat brain, rat liver, COS-7
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:500-1:1000	Hu, Ms, Rt
IHC-P	1:200	Hu, Ms, Rt

Background

ACSS2 (Acyl-CoA Synthetase Short-Chain Family Member 2) is a metabolic enzyme primarily localized in the cytoplasm, whose core function is to catalyze the conversion of acetate into acetyl-CoA, thereby connecting metabolism with lipid synthesis, energy production, and gene expression regulation. In its classical role, ACSS2 provides substrates for histone acetylation by generating acetyl-CoA, thus epigenetically regulating gene transcription; however, recent studies have revealed that its functions extend far beyond this. It can directly acetylate metabolic enzymes such as PAICS to influence purine synthesis and DNA repair, thereby regulating the senescence-associated secretory phenotype (SASP). Moreover, upon responding to oncogenic signals, ACSS2 can be phosphorylated, translocate to the nucleus, and utilize lactate as a substrate to synthesize lactyl-CoA, collaborating with KAT2A to catalyze histone lactylation, thereby promoting tumor immune evasion. Additionally, in alcoholic liver disease, the downregulation of ACSS2 epigenetically inhibits hepcidin expression, inducing ferroptosis and liver injury. These findings reveal ACSS2 as a critical metabolic-epigenetic hub that, through its substrate diversity and functional plasticity, plays a complex and important role in aging, inflammation, and tumor development and progression.

Picture

Western Blot

WB result of ACSS2 Recombinant Rabbit mAb
Primary antibody: ACSS2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Raji whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: Caco-2 whole cell lysate 20 µg
Lane 4: U-87 MG whole cell lysate 20 µg
Low expression control: Raji whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 78 kDa
Observed MW: 78 kDa

WB result of ACSS2 Recombinant Rabbit mAb
Primary antibody: ACSS2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Lane 3: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 78 kDa
Observed MW: 78 kDa

WB result of ACSS2 Recombinant Rabbit mAb
Primary antibody: ACSS2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 78 kDa
Observed MW: 78 kDa

WB result of ACSS2 Recombinant Rabbit mAb
Primary antibody: ACSS2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 78 kDa
Observed MW: 78 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human colon. Anti-ACSS2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-ACSS2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-ACSS2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-ACSS2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.