Acetyllysine Recombinant Rabbit mAb (SDT-R096)

Catalog Number: S0B0070 Application: WB, IHC-P Reactivity: Species Independent Conjugation: Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	Acetyllysine
Immunogen	N/A
Location	N/A
Clone Number	SDT-R096
Application	WB, IHC-P
Reactivity	Species Independent
Purification	Protein A
Concentration	0.5 mg/ml
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000
IHC-P	1:2000

Background

Acetylation is a dynamic post-translational modification that is attached to protein substrates by lysine acetyltransferases (KATs) and removed by lysine deacetylases (KDACs). While these enzymes are best characterized as histone modifiers and regulators of gene transcription, work in a number of systems highlights that acetylation is a pervasive modification and suggests a broad scope for KAT and KDAC functions in the cell [PMID: 26512033].

Picture

Western Blot

WB result of Acetyllysine Rabbit mAb Primary antibody:Acetyllysine Rabbit mAb at 1/1000 dilution Lane 1: untreated HeLa whole cell lysate 20 µg Lane 2: HeLa treated with Sodium butyrate(5mM, 24h) whole cell lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: Multiple kDa Observed MW: Multiple kDa

WB result of Acetyllysine Rabbit mAb Primary antibody: Acetyllysine Rabbit mAb at 1/1000 dilution Lane 1: mouse kidney lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: Multiple kDa Observed MW: Multiple kDa

WB result of Acetyllysine Rabbit mAb Primary antibody: Acetyllysine Rabbit mAb at 1/1000 dilution Lane 1: rat kidney lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: Multiple kDa Observed MW: Multiple kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human liver. Anti-Acetyllysine antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Acetyllysine antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Acetyllysine antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.