WB result of S-RMabMix™ Acetyllysine Rabbit mAb
Primary antibody: S-RMabMix™ Acetyllysine Rabbit mAb at 1/500 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple
Observed MW: Multiple
Exposure time: 180 s
S-RMabMixTM Acetyllysine Rabbit mAb
S-RMabMixTM Acetyllysine Rabbit mAb
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$110 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Acetyllysine |
| Synonyms | Kac, acetylation |
| Clone Number | S-R373 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IP |
| Reactivity | Species Independent |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:500 | |
| WB | 1:500 | |
| IP | 1:20 |
Background
Acetyllysine (or acetylated lysine) is an acetyl-derivative of the amino acid lysine. In proteins, the acetylation of lysine residues is an important mechanism of epigenetics. It functions by regulating the binding of histones to DNA in nucleosomes and thereby controlling the expression of genes on that DNA. Non-histone proteins are acetylated as well. Unlike the functionally similar methyllysine, acetyllysine does not carry a positive charge on its side chain. Histone acetyltransferases (HATs) catalyze the addition of acetyl groups from acetyl-CoA onto certain lysine residues of histones and non-histone proteins. Histone deacetylases (HDACs) catalyze the removal of acetyl groups from acetylated lysines.
Picture
Picture
Western Blot
Dot Blot
Dot blot result of S-RMabMix™ Acetyllysine Rabbit mAb
Lane 1: Modified peptide library (Acetyl lysine)
Lane 2: Unmodified peptide library control
Lane 3: Modified peptide library (Fumaryl lysine)
Lane 4: Modified peptide library (Crotonyl lysine)
Lane 5: Modified peptide library (Succinyl lysine)
Lane 6: Modified peptide library (Butyryl lysine)
Lane 7: Modified peptide library (L-lactyl lysine)
Primary antibody: S-RMabMix™ Acetyllysine Rabbit mAb at 1/500 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
