WB result of ABCC1/MRP1 Recombinant Rabbit mAb
Primary antibody: ABCC1/MRP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled SK-MEL-2 whole cell lysate 20 µg
Lane 2: unboiled HepG2 whole cell lysate 20 µg
Lane 3: unboiled PANC-1 whole cell lysate 20 µg
Lane 4: unboiled A549 whole cell lysate 20 µg
Lane 5: unboiled HeLa whole cell lysate 20 µg
Lane 6: unboiled BxPC-3 whole cell lysate 20 µg
Lane 7: unboiled SK-OV-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 171 kDa
Observed MW: 180-300 kDa
ABCC1/MRP1 Recombinant Rabbit mAb (SDT-4056-11)
ABCC1/MRP1 Recombinant Rabbit mAb (SDT-4056-11)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ABCC1 |
| Synonyms | Multidrug resistance-associated protein 1; ATP-binding cassette sub-family C member 1; Glutathione-S-conjugate-translocating ATPase ABCC1; Leukotriene C(4) transporter (LTC4 transporter); MRP |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P33527 |
| Clone Number | SDT-4056-11 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Mk |
| Positive Sample | SK-MEL-2, HepG2, PANC-1, A549, HeLa, BxPC-3, SK-OV-3, COS-7 |
| Purification | Protein A |
| Concentration | 0.5mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Mk |
| IHC-P | 1:1000 | Hu |
Background
ABCC1, also known as multidrug resistance-associated protein 1 (MRP1), is a 180–190 kDa integral membrane protein that belongs to the ATP-binding cassette (ABC) transporter superfamily. It contains two membrane-spanning domains, each with six α-helices, and two nucleotide binding domains, along with an additional third membrane-spanning domain. This protein functions as a multispecific organic anion transporter, with substrates including oxidized glutathione, cysteinyl leukotrienes, activated aflatoxin B1, glucuronides, and sulfate conjugates of steroid hormones and bile salts. It is highly conserved and expressed in various tissues, particularly in the lungs, spleen, testes, kidneys, placenta, thyroid, bladder, adrenal glands, and endothelium cells of the blood-brain barrier. ABCC1 plays a significant role in multidrug resistance of cancerous tumor cells by transporting chemotherapeutic drugs out of the cells. Additionally, it has been a point of interest in Alzheimer's disease research due to its ability to decrease β-amyloid accumulation in the brain.
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Western Blot
WB result of ABCC1/MRP1 Recombinant Rabbit mAb
Primary antibody: ABCC1/MRP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 171 kDa
Observed MW: 180-300 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti-ABCC1/MRP1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-ABCC1/MRP1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreas cancer. Anti-ABCC1/MRP1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-ABCC1/MRP1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human Squamous Cell Lung Cancer. Anti-ABCC1/MRP1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti-ABCC1/MRP1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
