Aass Recombinant Rabbit mAb (S-4170-22)

Catalog Number: S0B60023 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100µl

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Product Details

Product Specification

Host	Rabbit
Antigen	Aass
Synonyms	Alpha-aminoadipic semialdehyde synthase, mitochondrial; AASS
Immunogen	Synthetic Peptide
Location	Mitochondrion
Accession	Q9UDR5
Clone Number	S-4170-22
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt
Positive Sample	293T, HepG2, HUVEC, HeLa
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu
IHC-P	1:500-1:2000	Hu, Ms, Rt

Background

Aass (alpha-aminoadipic semialdehyde synthase, also known as lysine-ketoglutarate reductase/saccharopine dehydrogenase) is a bifunctional enzyme localized to the mitochondria and plays a critical role in the lysine degradation pathway. This enzyme consists of two independent domains: its N-terminal domain possesses lysine-ketoglutarate reductase activity, catalyzing the conversion of lysine and α-ketoglutarate into saccharopine; while its C-terminal domain exhibits saccharopine dehydrogenase activity, further converting saccharopine into α-aminoadipic semialdehyde, thereby completing the first two steps of lysine catabolism. Dysfunction of this gene directly leads to lysine metabolic disorders, resulting in hyperlysinemia. Notably, when the saccharopine dehydrogenase domain is specifically impaired, it causes abnormal accumulation of the metabolic intermediate saccharopine in the body, which subsequently disrupts mitochondrial homeostasis and affects organismal development.

Picture

Western Blot

WB result of Aass Recombinant Rabbit mAb
Primary antibody: Aass Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: HUVEC whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 102 kDa
Observed MW: 110 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human kidney. Anti-Aass antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver. Anti-Aass antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-Aass antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse kidney. Anti-Aass antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat kidney. Anti-Aass antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat liver. Anti-Aass antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.