WB result of AARS1 Rabbit pAb
Primary antibody: AARS1 Rabbit pAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 107 kDa
Observed MW: 110 kDa
AARS1 Rabbit Polyclonal Antibody
AARS1 Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | AARS1 |
| Synonyms | Alanine--tRNA ligase, cytoplasmic; Alanyl-tRNA synthetase (AlaRS); Protein lactyltransferase AARS1; Renal carcinoma antigen NY-REN-42; AARS |
| Location | Cytoplasm, Nucleus |
| Accession | P49588 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | MCF7, A549, HeLa, HepG2, Raji, NIH/3T3, C6 |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
AARS1 (Alanyl-tRNA synthetase 1) is a highly evolutionarily conserved class II aminoacyl-tRNA synthetase, whose core function is to catalyze the ligation of alanine to its corresponding tRNA(Ala), thereby providing critical quality control for protein synthesis. This enzyme accomplishes its task through a two-step process: first, it activates alanine using ATP to form alanyl-AMP; second, it transfers the activated alanine to tRNA (Ala). To ensure absolute translational fidelity, AARS1 is also equipped with a built-in editing or proofreading domain, which hydrolyzes and corrects mischarged amino acids, thus preventing the synthesis of toxic proteins caused by errors in genetic information translation. Although AARS1 is widely expressed throughout the body and primarily localizes to the cytoplasm, its dysfunction exhibits remarkable tissue specificity—particularly within the nervous system, where mutations in this gene have been definitively linked to several severe neurodegenerative diseases, such as Charcot-Marie-Tooth disease (CMT2N) and developmental epileptic encephalopathy (DEE29).
Picture
Picture
Western Blot
WB result of AARS1 Rabbit pAb
Primary antibody: AARS1 Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 107 kDa
Observed MW: 110 kDa
WB result of AARS1 Rabbit pAb
Primary antibody: AARS1 Rabbit pAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 107 kDa
Observed MW: 110 kDa
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-AARS1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in HepG2 cells. Anti-AARS1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in Raji cells. Anti-AARS1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
