WB result of AACT Recombinant Rabbit mAb
Primary antibody: AACT Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: human serum lysate 5 µg
Lane 2: human plasma lysate 5 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 60~70 kDa
AACT Recombinant Rabbit mAb (S-897-32)
AACT Recombinant Rabbit mAb (S-897-32)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | AACT |
| Synonyms | Alpha-1-antichymotrypsin, ACT, Cell growth-inhibiting gene 24/25 protein, Serpin A3 |
| Immunogen | Recombinant Protein |
| Location | Secreted |
| Accession | P01011 |
| Clone Number | S-897-32 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IP |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | null |
| IP | 1:50 | null |
Background
Alpha-1-antichymotrypsin (AACT), also known as SERPIN family A member 3 (SERPINA3), is a glycoprotein primarily synthesized in the liver and astrocytes, then secreted into surrounding tissues or blood, where it plays significant roles. AACT is capable of binding to mast cell chymase and neutrophil cathepsin G, thereby protecting cells and tissues from damage. In various conditions such as fibromyalgia, multiple sclerosis, chronic heart failure, Alzheimer's disease, and certain cancers, AACT levels are elevated and can serve as a diagnostic or prognostic biomarker.
Picture
Picture
Western Blot
IP
AACT Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating AACT in 0.4 mg human serum lysate.
Western blot was performed on the immunoprecipitate using AACT Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: human serum lysate 5 µg (Input)
Lane 2: AACT Rabbit mAb IP in human serum lysate
Lane 3: Rabbit monoclonal IgG IP in human serum lysate
Predicted MW: 48 kDa
Observed MW: 60~70 kDa
