WB result of 5HT1A Receptor Rabbit pAb
Primary antibody: 5HT1A Receptor Rabbit p Ab at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 46 kDa
Observed MW: 46 kDa
5HT1A Receptor Rabbit Polyclonal Antibody
5HT1A Receptor Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | 5HT1A |
| Synonyms | 5-hydroxytryptamine receptor 1A; 5-HT-1A; 5-HT1A; G-21; Serotonin receptor 1A; HTR1A; ADRB2RL1; ADRBRL1 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane, Cell projection |
| Accession | P08908 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | mouse brain, rat brain |
| Predicted Reactivity | Dg, Cz, Hr, Or, Gor |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms, Rt |
| IHC-P | 1:50 | Hu, Ms, Rt |
Background
5-HT1A, also known as the serotonin 1A receptor, is a G protein-coupled receptor that plays a significant role in regulating a variety of physiological processes and has been implicated in several pathologies, particularly in the realm of mental health1. It is highly expressed in the brain, particularly in regions such as the hippocampus, septum, and prefrontal cortex, and is known to modulate serotonin activity, which in turn influences mood, anxiety, and other behaviors. The 5-HT1A receptor functions as both a presynaptic autoreceptor, inhibiting the release of serotonin, and as a postsynaptic heteroreceptor, modulating the effects of serotonin in the brain. It is also found on the cell bodies and dendrites of neurons, where it can influence neuronal excitability and synaptic transmission.
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Picture
Western Blot
WB result of 5HT1A Receptor Rabbit pAb
Primary antibody: 5HT1A Receptor Rabbit p Ab at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 46 kDa
Observed MW: 46 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human liver. Anti-5HT1A antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hippocampus. Anti-5HT1A antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-5HT1A antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-5HT1A antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
