4E-BP1 Recombinant Rabbit mAb (S-4103-65)

Catalog Number: S0B6953 Application: WB, IHC-P, ICC Reactivity: Hu, Ms, Rt, Mk Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25µl 100µl

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Product Details

Product Specification

Host	Rabbit
Antigen	4E-BP1
Synonyms	Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1 (PHAS-I); EIF4EBP1
Immunogen	Synthetic Peptide
Location	Cytoplasm, Nucleus
Accession	Q13541
Clone Number	S-4103-65
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P, ICC
Reactivity	Hu, Ms, Rt, Mk
Positive Sample	Raji, HepG2, HeLa, MCF7, C2C12, NIH/3T3, mouse skeletal muscle, rat skeletal muscle, COS-7
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:5000	Hu, Ms, Rt, Mk
IHC-P	1:1000	Hu
ICC	1:500	Ms

Background

4E-BP1 (eukaryotic initiation factor 4E-binding protein 1) is a key regulator of protein translation and plays a crucial role in controlling the assembly of the eIF4F complex, which is essential for cap-dependent translation initiation. It is the most widely expressed and well-characterized member of the 4E-BP family in mammalian cells. When active, 4E-BP1 binds to eIF4E, preventing its interaction with eIF4G and thereby inhibiting the formation of the eIF4F complex, which is necessary for the translation of many oncogenic mRNAs with structured 5′-UTRs. This mechanism is critical in suppressing tumor development, as hyperactive eIF4E-dependent translation contributes to the upregulation of pro-tumorigenic genes in cancer cells. The activity of 4E-BP1 is regulated by various signaling pathways, including the PI3K/AKT/mTOR pathway, which can lead to its phosphorylation and inactivation, allowing eIF4E to promote translation. Reactivating 4E-BP1 through mTOR inhibition or other mechanisms has been shown to have tumor-suppressive effects.

Picture

Western Blot

WB result of 4E-BP1 Recombinant Rabbit mAb
Primary antibody: 4E-BP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Raji whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Low expression control: Raji whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 13 kDa
Observed MW: 15-20 kDa

WB result of 4E-BP1 Recombinant Rabbit mAb
Primary antibody: 4E-BP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Lane 3: mouse skeletal muscle lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 13 kDa
Observed MW: 15-20 kDa

WB result of 4E-BP1 Recombinant Rabbit mAb
Primary antibody: 4E-BP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat skeletal muscle lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 13 kDa
Observed MW: 15-20 kDa

WB result of 4E-BP1 Recombinant Rabbit mAb
Primary antibody: 4E-BP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 13 kDa
Observed MW: 15-20 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human kidney. Anti-4 E-BP1antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-4 E-BP1antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-4 E-BP1antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunocytochemistry

ICC shows positive staining in NIH/3T3 cells. Anti-4E-BP1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).