2μg (R: reducing condition, N: non-reducing condition).
4-1BB/TNFRSF9 His Tag Protein, Human
4-1BB/TNFRSF9 His Tag Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | TNFRSF9, 4-1BB, CD137, CDw137, ILA |
| Accession | Q07011 |
| Amino Acid Sequence | Leu24-Gln186, with C-terminal His |
| Expression System | HEK293 |
| Molecular Weight | 25-33kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4. |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | Melero I, et al. (2008) Multi-layered action mechanisms of CD137 (4-1BB)-targeted immunotherapies. Trends Pharmacol Sci. 29(8): 383-90. Thum E, et al. (2009) CD137, implications in immunity and potential for therapy. Front Biosci. 14: 4173-88. |
Background
CD137 (also known as 4-1BB) is a surface co-stimulatory glycoprotein originally described as present on activated T lymphocytes, which belongs to the tumor necrosis factor (TNF) receptor superfamily. CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. In addition, CD137 expression is found on dendritic cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation. The best characterized activity of CD137 is its costimulatory activity for activated T cells. 4-1BB signaling either by binding to 4-1BBL or by antibody ligation delivers signals for T-cell activation and growth, as well as monocyte proliferation and B-cell survival, and plays an important role in the amplification of T cell-mediated immune responses.
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SDS-PAGE
SEC-HPLC
ELISA
Immobilized 4-1BB/TNFRSF9 His Tag Protein, Human (Cat. No. UA010796)at 2.0μg/mL (100μL/well) can bind Human 4-1BB Ligand/TNFSF9 Trimer Protein with EC50 of 1.32-2.12ng/mL.
Immobilized 4-1BB/TNFRSF9 His Tag Protein, Human (Cat. No. UA010796)at 2.0μg/mL (100μL/well) can bind Anti-Human CD137 Monoclonal Antibody with EC50 of 23.19-25.51ng/mL.
