4-1BB/CD137/TNFRSF9 Recombinant Rabbit mAb (S-4989)

Catalog Number: S0B6899 Application: WB, IHC-P Reactivity: Hu Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25µl 100µl

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Product Details

Product Specification

Host	Rabbit
Antigen	4-1BB/CD137/TNFRSF9
Synonyms	Tumor necrosis factor receptor superfamily member 9; 4-1BB ligand receptor; CDw137; T-cell antigen 4-1BB homolog; T-cell antigen ILA; ILA
Location	Cell membrane
Accession	Q07011
Clone Number	S-4989
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu
Positive Sample	HeLa, Jurkat, HDLM-2
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu
IHC-P	1:100	Hu

Background

CD137, also known as 4-1BB, is a co-stimulatory molecule expressed on the surface of activated T cells and natural killer cells. It plays a crucial role in enhancing the immune response by providing additional signals that promote T cell proliferation, survival, and cytokine production. CD137 is a member of the tumor necrosis factor receptor superfamily and its ligand, CD137L, is primarily found on antigen-presenting cells. The interaction between CD137 and CD137L helps to sustain the activation of T cells and supports the development of long-lasting immune responses, making it an important target for immunotherapy in cancer treatment and other diseases where modulation of the immune system is beneficial.

Picture

Western Blot

WB result of 4-1BB/CD137/TNFRSF9 Recombinant Rabbit mAb
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: 4-1BB/CD137/TNFRSF9 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: HDLM-2 whole cell lysate 20 µg
Negative control: HeLa whole cell lysate; Jurkat whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 32, 38 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human Hodgkin lymphoma. Anti-4-1BB/CD137/TNFRSF9 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human thymus. Anti-4-1BB/CD137/TNFRSF9 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human tonsil. Anti-4-1BB/CD137/TNFRSF9 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.