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    3D Cell Spheroid Hydrogel Kit

    3D Cell Spheroid Hydrogel Kit

    Catalog Number: abs90548 Brand: Absin
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    $244 USD
    $244 USD
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    Product Details

    Product Specification

    Usage 1. Reagent preparation steps:
    1 、 Gel At 37℃ water bath preheating 10 min Confirm complete melting, and place before use. 37℃ The water bath can reduce the viscosity of the colloid.
    2 、 Buffer (1X) ： Use 4℃ DMEM The culture medium will Buffer (10X) Dilute into Buffer (1X) Place in the refrigerator or on crushed ice before use.

    II. 3D cell sphere - Droplet Culture Steps:
    All steps were performed in a sterile operating bench, and the operating steps were as follows:
    1 Prepared using complete culture medium containing 2×105~107cells/mL cell suspension and Gel According to 1 ： 1 Uniformly mix to prepare a cell suspension, so that the final cell density is 105~107cells/mL , get Gel Cell suspension.
    Note 1 ： A Gel The solution is below 22℃ It is easy to form a gel and melt; please proceed promptly.
    Note 2 If different hardness requirements are required, please refer to the colloid hardness adjustment ratio. Q&A 。
    2 , take 20 ~ 40 μL  Gel The cell suspension is dropped into the cell culture plate, and the culture plate is carefully transferred to crushed ice or 4℃ Inside the refrigerator, glue droplets 5 min Internal gel formation.
    Note 3 It is recommended that the gel droplets be as small and round as possible.
    3 After the gel droplets form gel, they are added to the culture plate along the sidewall of the well. 1 mL pre-cooled Buffer (1X) Cover with glue droplets and let stand on crushed ice. 15 min Crosslinking.
    4 After crosslinking, Buffer (1X) Aspirate out and replace with complete medium suitable for cell growth.
    5 Place the cell culture plate in 37 °C Culture in carbon dioxide incubator 5~14 Days, and observe the formation of cell spheres. Perform the medium change operation according to the normal medium change frequency.

    III. Sols and Collection of Cell Spheres:
    If required, the cell recovery solution can be ordered separately (item number: abs90549 ）
    1 Reagent preparation:
    cell recovery solution (1X) : Use 4℃ PBS (1X) Cell recovery solution (10X) Diluted celluloblast recovery solution (1X) 。
    2 Collecting Cell Spheres:
    （ 1 Carefully aspirate and remove the culture medium, and use... PBS (1X) Perform cleaning. will PBS Remove by suction and add 1 mL cell recovery solution (1X) Cover the gel droplets and react at room temperature. 5 min 。
    （ 2 Gently blow the gel droplets with a pipette until the gel droplets are completely dissolved.
    （ 3 The solution containing cell spheres is drawn into a centrifuge tube to... 250g centrifuge 5 min The supernatant was removed and the pelleted cell spheres were collected for subsequent experimental analysis.
    3 Collection of single cells:
    （ 1 Before separating the cell mass, follow the steps described above. “ Sols and Collection of Cell Spheres ” The method is used to perform the operation.
    （ 2 ) Add 0.25% Pancreatin ( abs47014938 ) and the collected cell spheres in 37°C Mixing reaction. use 1 mL The pipette gently blows the cell pellet until the cell spheres are completely digested.
    3 After the cell spheres are completely digested, add... 3 double volume PBS (1X) , with 250g centrifuge 5 min The supernatant was removed and the pelleted single cells were collected for subsequent experimental analysis.
    Description 3D The cell sphere hydrogel kit contains a complete set of colloids and reagents, the polymer colloids of which can quickly form hydrogels. 3D Applications related to cell culture and microenvironment research. This kit is convenient to operate and can regulate colloidal hardness according to the characteristics of the source tissue for various cell culture tests.
    Product Composition:
    Product Name 5mL 10mL Preserve
    Gel 5mL 10mL -20℃
    Buffer(10X) 25mL 50mL -20℃
    Note:
    1 This product is shipped with blue ice. Please transfer it to the appropriate temperature as soon as possible after receiving it according to the product's storage requirements. This product is recommended to be used immediately after thawing. If not for immediate use, please repackage and store.
    2 Please use this product within six months after opening.
    Applicable cell types:
    1 Normal cell lines
    2 tumor cell lines,
    Storage Temp. Store at -20℃ for 12 months.
    Applications 3D cell sphere culture test; 3D cell sphere drug screening platform; Cancer cell line invasion assay; Hydrogel Coating Test