10x Kinase Buffer C
10x Kinase Buffer C
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$25 USD
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$25 USD
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Product Details
Product Details
Product Specification
| Physical Appearance | Liquid |
| Stability & Storage | For long term storage, keep 10X buffer at -20°C. If buffer will be continually used, it is recommended that the 10X buffer be kept at 4°C for 1-2 weeks. For optimal storage, aliquot buffer into smaller quantities after centrifugation and store at recommended temperature. For most favorable |
Background
Kise Buffer can be used to assay protein kise activity.
Components
250 mM Tris-HCl (pH 7.5), 50 mM beta-glycerophosphate, 20 mM dithiothreitol (DTT), 1 mM 3VO4, 100 mM MgCl2
Protocol
1. Thaw 10X buffer at 24-30°C, mixing end-over-end.
2. Dilute the 10X buffer to the desired concentration using ddH2O.
Guidelines
Kise Buffer must be supplemented with ATP.
