KGF/FGF-7 Protein, Human

Catalog Number: UA040561 Brand: UA BIOSCIENCE

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$130 USD

Size: 10μg 50μg 100μg 500μg 1mg

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Product Details

Product Specification

Species	Human
Synonyms	Fibroblast growth factor 7, FGF-7, Heparin-binding growth factor 7 (HBGF-7), Keratinocyte growth factor
Accession	P21781
Amino Acid Sequence	Cys32-Thr194
Expression System	CHO
Molecular Weight	24-32 kDa (Reducing)
Purity	>95% by SDS-PAGE & HPLC.
Endotoxin	<0.1EU/μg
Conjugation	Unconjugated
Tag	No Tag
Physical Appearance	Lyophilized Powder
Storage Buffer	PBS, pH7.4
Reconstitution	Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage	·12 months from date of receipt, lyophilized powder stored at -20 to -80℃. ·3 months, -20 to -80℃ under sterile conditions after reconstitution. ·1 week, 2 to 8℃ under sterile conditions after reconstitution. ·Please avoid repeated freeze-thaw cycles.
Reference	Am J Transplant. 2021 Sep;21(9):2950-2963. Development. 1999 Feb;126(3):547-54.

Background

Human fibroblast growth factor 7 (FGF-7) is also known as keratinocyte growth factor (KGF). It is a secreted, heparin-binding glycoprotein of 194 amino acids that belongs to the FGF family. FGF-7 is produced exclusively by mesenchymal cells and acts in a paracrine manner on epithelial cells expressing the splice variant FGFR2-IIIb. Upon ligand binding, the receptor dimerizes and initiates downstream signaling through the MAPK/ERK, PI3K/AKT and PLCγ pathways, leading to proliferation, migration, differentiation and survival of epithelial cells. Expression of FGF-7 is strongly up-regulated after skin, lung, kidney or gastrointestinal injury by inflammatory cytokines such as IL-1 and TNF-α, making it a key mediator of wound healing and tissue repair.

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Bioactivity

Measured in a cell proliferation assay using Hacat cells.

KGF/FGF-7 Protein, Human (Cat#UA040561) maintains lung cancer organoid growth well during multiple passages and long-term culture processes.

KGF/FGF-7 Protein, Human (Cat#UA040561) maintains bud tip progenitor organoid growth well during multiple passages and long-term culture processes.

Determined by a cell proliferation assay using 4MBr-5 cells. The expected EC50 for this effect is 3.6-8.7ng/ml.

iPSC‑derived islet organoids were cultured in medium containing Activin (UA040418), EGF (UA040575), and FGF‑7 (UA040561).

Immunofluorescence staining showed co‑localization of PDX1 (purple, nuclear positive) and insulin (green, cytoplasmic granular) in iPSC‑derived pancreatic islet organoids, confirming successful differentiation into functional beta‑like cells.