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CRBN/DDB1/CUL4A/RBX1 Protein, Human

CRBN/DDB1/CUL4A/RBX1 Protein, Human

Catalog Number: UA080275 Brand: UA BIOSCIENCE
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Product Details

Product Specification


Species Human
Accession Q96SW2、Q16531、Q13619、P62877
Amino Acid Sequence

M1-L442 end(CRBN)
M1-H1140 end(DDB1)
A2-A759 end(Cul4a)
M1-H108 end(RBX1)
N-His-TEV-Avi (CRBN)/No tag (DDB1)/ No tag (Cul4a)/No tag
(RBX1)
Expression System Baculovirus-InsectCells
Molecular Weight

55.3 KDa/126.9 KDa/87.6 KDa/12.2 KDa
Conjugation Unconjugated
Tag Avi Tag, His Tag
Physical Appearance Liquid
Storage Buffer

50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol, 1 mM DTT
Stability & Storage

-80℃

Guidelines

Please use rapid thawing with running water to thaw the protein samples.

For complete recovery, mix well and spin before use.

Product must not be stored in diluted solutions.

Aliquots below 10μl are not advisable.

Avoid repetitive freeze-thaw cycles!

Picture

Bioactivity

Lane M: Marker Lane 1: Bodipy-Ub + UBE1 + Ubch5b + CRBN/DDB1/CUL4A/RBX1 with ATP
Lane 2: Bodipy-Ub + UBE1+ Ubch5b with ATP
Lane 3: Bodipy-Ub + UBE1+ Ubch5b without ATP
The ubiquitin conjugating activity of CRBN/DDB1/CUL4A/RBX1 was validated through its ability to catalyse
the generation of polyubiquitin chains in the presence of the E1 activating enzyme UBE1, the E2 conjugating
enzyme Ubch5b and Bodipy-ubiquitin. Incubation of CRBN/DDB1/CUL4A/RBX1 for 60 minutes at 37˚C in the
presence of Bodipy-ubiquitin, UBE1, Ubch5b and ATP (Lane 1) was compared alongside two control reactions
with either CRBN/DDB1/CUL4A/RBX1 (Lane 2) or CRBN/DDB1/CUL4A/RBX1&ATP (Lane 3) excluded from
the reaction. Ubiquitin conjugates were identified by the migration of the Bodipy-ubiquitin band and these were
observed only in the presence of ATP and CRBN/DDB1/CUL4A/RBX1.

SDS-PAGE

Lane M: Marker
Lane 1: 2 μg, reduced
Lane 2: 2 μg, non-reduced