Standard curve
Example of CCL2/JE/MCP-1 standard curve in Assay Diluent .
Mouse CCL2/JE/MCP-1 Single Plex Kit (Flow Cytometry Bead Assay)
Mouse CCL2/JE/MCP-1 Single Plex Kit (Flow Cytometry Bead Assay)
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Product Details
Product Details
Product Specification
| Antigen | CCL2/JE/MCP-1 |
| Reactivity | Mouse |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8°C as supplied. |
Kit
| Precision | Intra-assay:； Inter-assay: |
| Sample type | Serum; Plasma-EDTA; Plasma-Heparin; Plasma-Citrate; Cell Culture Supernatant |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 76.91 pg/mL |
| Range | 38.12 pg/mL-170000 pg/mL |
| Recovery | Serum、plasma and cell culture supernatant: 70%~130% |
| Assay time | 70 minutes |
| Species reactivity | Mouse |
Background
Mouse CCL2, also known as JE or MCP-1 (Monocyte Chemoattractant Protein-1), is a cytokine that belongs to the CC chemokine family. It is primarily produced by monocytes, macrophages, and various other cell types in response to inflammatory stimuli. CCL2 plays a crucial role in the recruitment and activation of monocytes, memory T cells, and dendritic cells to sites of inflammation, thereby contributing to the immune response. This protein is involved in various physiological and pathological processes, including the regulation of immune cell migration during inflammation, the development of atherosclerosis, and the progression of certain cancers. Its expression and activity are tightly regulated, and dysregulation of CCL2 has been implicated in numerous diseases, making it a potential target for therapeutic interventions.
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Picture
CBA
Quantifiction of Mouse Splenocytes
Stimulated sample: Mouse Splenocytes were stimulated with 20 ng/mL PMA and 0.2 µg/mL lonomycin for 16h, and the average concentration of CCL2/JE/MCP-1 in the supernatant was measured to be 1654.71 pg/mL.
Unstimulated sample: Mouse Splenocytes were cultured for 16h, and the average concentration of CCL2/JE/MCP-1 in the supernatant was measured to be 55.91 pg/mL.
Linearity
The high-concentration mouse CCL2/JE/MCP-1 protein was serially diluted using serum, plasma-EDTA, plasma-heparin, plasma-citrate(CD-1 (ICR)、C57BL/6、BALB/c) and cell culture supernatant matrices respectively. Then, the concentration of mouse CCL2/JE/MCP-1 was determined and interpolated based on the target standard curve, and the sample dilution was corrected.
Protocol Diagram
