Standard curve
Example of IL-13 standard curve in Assay Diluent .
Human IL-13 Single Plex Kit (Flow Cytometry Bead Assay)
Human IL-13 Single Plex Kit (Flow Cytometry Bead Assay)
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Product Details
Product Details
Product Specification
| Antigen | IL-13 |
| Reactivity | Human |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8°C as supplied. |
Kit
| Precision | Intra-assay: 2.46%； Inter-assay: 3.01% |
| Sample type | Serum; Plasma; Cell culture supernatant |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 0.15 pg/mL |
| Range | 0.55 - 4500 pg/mL |
| Recovery | 75.53% - 101.13% |
| Assay time | 70 minutes |
| Species reactivity | Human |
Background
IL-13 is a 132-amino-acid nonglycosylated cytokine that shares common features with IL-4. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils. IL-13 signals through a receptor complex containing IL-13 R alpha 1 and IL-4 R alpha. This complex also functions as the type 2 IL-4 receptor. IL-13 bound IL-13 R alpha 2 with high affinity and can directly promote tumor cell invasiveness and the development of tissue fibrosis. Nevertheless, like IL-4, IL-13 inhibits macrophage production of TNF, IL-1β, and pro-inflammatory chemokines, but can upregulate the synthesis of IL-12 by DCs and macrophages.
Picture
Picture
CBA
Recovery
Within the detection range, the human IL-13 protein was added to different matrices at high, medium, low concentrations.
The matrices used in these experiments were diluted 2-fold with the assay buffer before the addition of the cytokine proteins.
The results were compared with those of samples that had the same concentration of cytokines added in the assay buffer.
Quantifiction of human PBMC
Stimulated sample: Human peripheral blood mononuclear cells (PBMCs) were stimulated with 5 μg/mL PHA for 24h, and the average concentration of IL-13 in the supernatant was measured to be 143.62 pg/mL.
Unstimulated sample: Human PBMCs were cultured for 24h, and the average concentration of IL-13 in the supernatant was measured to be 0.38 pg/mL.
Linearity
The high-concentration human IL-13 protein was serially diluted using plasma, serum and cell culture supernatant matrices respectively.
The matrices used in these experiments were diluted 2-fold with the assay buffer before the addition of the cytokine proteins.
Then, the concentration of human IL-13 was determined and interpolated based on the target standard curve, and the sample dilution was corrected.
Protocol Diagram
