Monkey E-Selectin/CD62E OneStep ELISA Kit

Monkey E-selectin (CD62E, ELAM-1) is a 107–115 kDa type I transmembrane glycoprotein of the selectin family, structurally composed of an extracellular C-type lectin domain (responsible for calcium-dependent carbohydrate recognition), an EGF-like domain, and six short consensus repeats, followed by a transmembrane region and a short cytoplasmic tail. Cynomolgus monkey E-selectin shares 96% amino acid identity with human E-selectin, making it a highly relevant translational model. E-selectin is expressed exclusively on activated endothelial cells, with peak induction at approximately 4 hours after stimulation by pro-inflammatory cytokines (TNF-α, IL-1β), followed by rapid decline within 24 hours. It mediates the initial tethering and rolling of leukocytes (neutrophils, monocytes, memory T cells) along the vascular endothelium by binding to sialylated/fucosylated ligands such as Sialyl Lewis X (CD15s) and PSGL-1 (CD162). Key binding residues identified by crystallography (PDB 1g1t) include Y48, N82, N83, E92, Y94, R97, N105, E107, E80, E98, and D106. After peak expression, E-selectin is proteolytically cleaved into soluble E-selectin, which circulates in blood and serves as a biomarker of endothelial activation.