WB result of GAPDH Recombinant Rabbit mAb
Primary antibody: GAPDH Recombinant Rabbit mAb at 1/200000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: MDA-MB-231 whole cell lysate 20 µg
Lane 4: SK-OV-3 whole cell lysate 20 µg
Lane 5: PC-3 whole cell lysate 20 µg
Lane 6: HEK-293 whole cell lysate 20 µg
Lane 7: A549 whole cell lysate 20 µg
Lane 8: HepG2 whole cell lysate 20 µg
Lane 9: PANC-1 whole cell lysate 20 µg
Lane 10: HT-29 whole cell lysate 20 µg
Lane 11: Ramos whole cell lysate 20 µg
Lane 12: Jurkat whole cell lysate 20 µg
Lane 13: K562 whole cell lysate 20 µg
Lane 14: A431 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 36 kDa
S-RMab® GAPDH Recombinant Rabbit mAb (S-240-147)
S-RMab® GAPDH Recombinant Rabbit mAb (S-240-147)
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | GAPDH |
| Synonyms | Glyceraldehyde-3-phosphate dehydrogenase |
| Location | Cytoplasm, Cytoskeleton, Nucleus |
| Accession | P04406、P16858 |
| Clone Number | S-240-147 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt, Zf, Mk, Ck |
| Predicted Reactivity | Hm (95%), Mi (95%), Pg (95%), Sq (95%), Dg (95%), Ct (95%), Sh (95%), Qu (95%), Bv (95%), Ck (95%) |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:10000-1:200000 | Hu, Ms, Rt, Zf |
| IHC-P | 1:4000 | Hu, Ms, Rt |
| ICC | 1:1000 | Hu, Ms, Rt |
Background
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a ubiquitous and highly conserved multifunctional protein that plays a central role in cellular metabolism, primarily catalyzing the sixth step of glycolysis where it converts glyceraldehyde-3-phosphate into 1,3-bisphosphoglycerate while simultaneously reducing NAD+ to NADH. Beyond its canonical function in energy production, GAPDH exhibits remarkable "moonlighting" activities depending on its subcellular localization, participating in diverse processes such as DNA repair, transcriptional regulation, vesicular transport, and the initiation of apoptosis under stress conditions like oxidative damage or nitrosative stress. Due to its constitutive and relatively stable expression across various tissues and experimental conditions, GAPDH has historically been widely used as a loading control in techniques like Western blotting and RT-PCR, although recent studies caution against this practice without validation since its levels can fluctuate significantly in response to specific metabolic states or pathological conditions. Structurally, it typically functions as a homotetramer, and its involvement in several neurodegenerative diseases, including Huntington's and Alzheimer's, highlights its critical importance in maintaining cellular homeostasis and its potential as a therapeutic target.
Picture
Picture
Western Blot
WB result of GAPDH Recombinant Rabbit mAb
Primary antibody: GAPDH Recombinant Rabbit mAb at 1/200000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: RAW264.7 whole cell lysate 20 µg
Lane 3: EL4 whole cell lysate 20 µg
Lane 4: C2C12 whole cell lysate 20 µg
Lane 5: Neuro-2a whole cell lysate 20 µg
Lane 6: mouse brain lysate 20 µg
Lane 7: mouse colon lysate 20 µg
Lane 8: mouse liver lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 36 kDa
WB result of GAPDH Recombinant Rabbit mAb
Primary antibody: GAPDH Recombinant Rabbit mAb at 1/200000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: PC-12 whole cell lysate 20 µg
Lane 3: rat brain lysate 20 µg
Lane 4: rat colon lysate 20 µg
Lane 5: rat liver lysate 20 µg
Lane 6: rat heart lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 36 kDa
WB result of GAPDH Recombinant Rabbit mAb
Primary antibody: GAPDH Recombinant Rabbit mAb at 1/200000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Lane 2: zebrafish lysate 20 µg
Lane 3: chicken liver lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 36 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human liver. Anti-GAPDH antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-GAPDH antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human bladder cancer. Anti-GAPDH antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-GAPDH antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-GAPDH antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-GAPDH antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-GAPDH antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
