| Host | Mouse |
| Antigen | ALPHA SMOOTH MUSCLE ACTIN |
| Synonyms | Alpha-actin-2, ACTA2, SMA |
| Immunogen | N/A |
| Location | Cytoplasm, Cytoskeleton |
| Accession | P62736 |
| Clone Number | SDT-R142 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG2a |
| Application | WB, IHC-P, ICC, ICFCM, IP |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.4 mg/ml |
| Tag | N/A |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
| application | dilution | species |
| WB | 1:1000-1:4000 | null |
| IHC-P | 1:4000 | null |
| ICC | 1:100 | null |
| ICFCM | 1:400 | null |
| IP | 1:40 | null |
α-smooth muscle actin, encoded by ACTA2, is expressed in abundance in vascular smooth muscle, comprising 50–70% of total actin, with the remainder composed of β-cytoplasmic and γ-actins. Whereas α-smooth muscle expression is normally restricted to smooth muscle cells, it can also be expressed in nonmuscle cells, most notably myofibroblasts that use cell traction forces to remodel extracellular matrix.
WB result of alpha smooth muscle Actin mouse mAb
Primary antibody: alpha smooth muscle Actin mouse mAb at 1/4000 dilution
Lane 1: mouse skeletal muscle lysate 5 µg
Lane 2: mouse stomach lysate 5 µg
Negative control: mouse skeletal muscle lysate
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42kDa
Observed MW: 42kDa
WB result of alpha smooth muscle Actin mouse mAb
Primary antibody: alpha smooth muscle Actin mouse mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42kDa
Observed MW: 42kDa
Flow cytometric analysis of HeLa cells labelling alpha smooth muscle Actin antibody at 1/400 (0.1 μg) dilution/ (red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
alpha smooth muscle Actin Mouse mAb at 1/40 dilution (1 µg) immunoprecipitating alpha smooth muscle Actin in 0.4 mg NIH/3T3 whole cell lysate.
Western blot was performed on the immunoprecipitate using alpha smooth muscle Actin Mouse mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: NIH/3T3 whole cell lysate 20 µg (Input)
Lane 2: alpha smooth muscle Actin Mouse mAb IP in NIH/3T3 whole cell lysate
Lane 3: Mouse monoclonal IgG2a IP in NIH/3T3 whole cell lysate
Predicted MW: 42 kDa
Observed MW: 42 kDa
IHC shows positive staining in paraffin-embedded human colon. Anti-alpha smooth muscle Actin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostate. Anti-alpha smooth muscle Actin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-alpha smooth muscle Actin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-alpha smooth muscle Actin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric carcinoma. Anti-alpha smooth muscle Actin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-alpha smooth muscle Actin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-alpha smooth muscle Actin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
ICC shows positive staining in HeLa cells. Anti-alpha smooth muscle Actin antibody was used at 1/100 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI. Counterstain with tubulin (red).
