Flow cytometric analysis of CD152 expression in activated human PBMC (human peripheral blood mononuclear cell). PHA-activated (2 days) human PBMC were fixed with 4% PFA and permeabilization with the 0.1% Tween and then were stained with either Mouse IgG2a, κ Isotype Control (left panel) or SDT Mouse Anti-Human CD152 Antibody at 1:200 dilution (1μg) (right panel). Goat Anti-Mouse IgG Alexa Fluor® 647 was used as the secondary antibody. Then cells were stained with CD3 - Brilliant Violet 421™ Antibody separately. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software
Mouse Anti-Human CD152 Antibody (BNI3)
Mouse Anti-Human CD152 Antibody (BNI3)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD152 |
| Location | Cell membrane |
| Accession | P16410 |
| Clone Number | BNI3 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a,k |
| Application | ICFCM |
| Reactivity | Hu |
| Positive Sample | PHA-activated (2 days) human PBMC |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2-8 °C as supplied |
Dilution
| application | dilution | species |
| ICFCM | 1:200 | Hu |
Background
CD152, also known as CTLA-4 (cytotoxic T-lymphocyte-associated protein 4), is a protein receptor that functions as an immune checkpoint. It is a member of the immunoglobulin superfamily and is expressed on the surface of T cells. The CD152 gene consists of 4 exons and encodes a protein with a signal peptide, an Ig-like domain, a transmembrane region, and a cytoplasmic domain. CD152 plays an essential role in regulating T cell activation by binding to its ligands CD80 and CD86, which are expressed on antigen-presenting cells. This binding can inhibit the over-activation of T cells, thus maintaining immune homeostasis.
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