TRKA titration in HTRF assay
TRKA(NTRK1) Protein
TRKA(NTRK1) Protein
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$767 USD
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Product Details
Product Details
Product Specification
| Species | Human |
| Accession | X5DR71 |
| Amino Acid Sequence |
C436-G790(end) CGRRNKFGINRPAVLAPEDGLAMSLHFMTLGGSSLSPTEGKGSGLQGHIIENPQYFSDACVHHIKRRDIVLKWELGEGAFGKVFLAECHNLLPEQDKMLVAVKALKEASESARQDFQREAELLTMLQHQHIVRFFGVCTEGRPLLMVFEYMRHGDLNRFLRSHGPDAKLLAGGEDVAPGPLGLGQLLAVASQVAAGMVYLAGLHFVHRDLATRNCLVGQGLVVKIGDFGMSRDIYSTDYYRVGGRTMLPIRWMPPESILYRKFTTESDVWSFGVVLWEIFTYGKQPWYQLSNTEAIDCITQGRELERPRACPPEVYAIMRGCWQREPQQRHSIKDVHARLQALAQAPPVYLDVLG |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | 66.4 kDa |
| Purity | >80% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | 50mM Tris, 150mM NaCl, 1mM DTT, 10% glycerol, 0.05% Brij35, pH7.5 |
| Stability & Storage |
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws
cycles.
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Bioactivity
The TRKA activity was detected using HTRF technology. The reaction was performed by incubating the TRKA protein , ATP and substrate at 25℃ for 60 min, adding the beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.
SDS-PAGE
SEC-HPLC
