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Rat IL-6 High Sensitivity ELISA Kit

Rat IL-6 High Sensitivity ELISA Kit

Catalog Number: abs553804 Application: ELISA Reactivity: Rat Conjugation:
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Regular price $450 USD
Regular price Sale price $450 USD
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Product Details

Product Specification

protein IL-6
Species Reactivity Rat
Source Rat
Description

Detection principle:

This kit uses double antibody sandwich ELISA Technologies. Specificity anti- Rat IL-6 capture antibody Pre-coated on high affinity enzyme plates. The standard substance and the sample to be tested are sequentially added to the well of the enzyme label plate , Thoroughly shake and mix, and then put it at room temperature 2 Hours of incubation process, present in the sample IL-6 Binding to solid phase antibodies. After thorough washing to remove free and unbound components, biotinylated detection antibody is added for incubation. After washing again, horseradish peroxidase-labeled streptavidin was added (Streptavidin-HRP , SA-HRP) 。 After washing, the signal enhancer was added for incubation, and after washing to remove unbound material, it was added again SA-HRP 。 After washing again, add TMB The chromogenic substrate is incubated at room temperature in the dark from light to develop color. The depth of color reaction and the sample IL-6 The concentration is positively correlated. Add a stop solution to stop the reaction, and use a microplate reader to 450 nm Detection wavelength ( Correction wavelength 570-630 nm) The absorbance values were determined under conditions.

 

Test Type: Double antibody sandwich method

 

Form: Pre-coating 96 Orifice plate

 

Test Sample Type: Cell supernatant, serum, plasma

 

Sample Load: 100 μl

 

Kit Components: Pre-coating 96 Well plate, standard, biotin labeled IL-6 Test antibody, standard dilution, test buffer, signal enhancer, signal enhancer dilution, TMB A chromogenic substrate, a wash liquid, a stop liquid, SA-HRP , a copy of the sealing film and instructions.

 

Sensitivity: 1.80 pg/mL

 

Detection range: 7.81-500 pg/mL

 

Recovery Range: 95-106%

 

Save Method: 2-8℃

 

Standard graph:

 

 

Background:

Interleukin 6(IL-6) It is a cytokine with both pro-inflammatory and anti-inflammatory properties. In humans, it is composed of IL-6 Genetic encoding. During infection and after trauma, especially inflammation caused by burns or other tissue damage, IL-6 Will be by T Cells and macrophages secrete, promoting the immune response. Studies have shown that mice need to resist Streptococcus pneumoniae infection IL-6 Participation, describing IL-6 Plays a role in fighting infections.

IL-6 Inflammatory and autoimmune disease processes can be promoted in many diseases such as diabetes, atherosclerosis, depression, Alzheimer's disease, systemic lupus erythematosus, multiple myeloma, prostate cancer, Behcet's disease and rheumatoid arthritis. later period / Patients with metastatic cancer have higher levels in their blood IL-6 。