Product Details
Product Details
Product Specification
| Species Reactivity | Mouse |
| Synonym | C9orf26, C9orf26chromosome 9 open reading frame 26 (NF-HEV), DKFZp586H0523, DVS27, DVS27-related protein, IL1F11, IL-1F11, IL33, IL-33, interleukin 33, Interleukin-1 family member 11, interleukin-33, NFHEV, NF-HEV, NF-HEVNFEHEV, Nuclear factor from high endothelial venules, RP11-575C20.2 |
| Description |
Detection principle: This kit uses double antibody sandwich ELISA Technologies. Specificity anti- Mouse IL-33 capture antibody Pre-coated on high affinity enzyme plates. The standard substance and the sample to be tested are sequentially added to the well of the enzyme label plate , Thoroughly shake and mix, and then put it at room temperature 2 Hours of incubation process, present in the sample IL-33 Binding to solid phase antibodies. After thorough washing to remove free and unbound components, biotinylated detection antibody is added for incubation. After washing again, horseradish peroxidase-labeled streptavidin was added (Streptavidin-HRP , SA-HRP) 。 After washing, the signal enhancer was added for incubation, and after washing to remove unbound material, it was added again SA-HRP 。 After washing again, add TMB The chromogenic substrate is incubated at room temperature in the dark from light to develop color. The depth of color reaction and the sample IL-33 The concentration is positively correlated. Add a stop solution to stop the reaction, and use a microplate reader to 450 nm Detection wavelength ( Correction wavelength 570-630 nm) The absorbance values were determined under conditions.
Test Type: Double antibody sandwich method
Form: Pre-coating 96 Orifice plate
Test Sample Type: Cell supernatant, serum, plasma
Sample Load: 100 μl
Kit Components: Pre-coating 96 Well plate, standard, biotin labeled IL-33 Test antibody, standard dilution, test buffer, signal enhancer, signal enhancer dilution, TMB A chromogenic substrate, a wash liquid, a stop liquid, SA-HRP , a copy of the sealing film and instructions.
Sensitivity: 0.26 pg/mL
Detection range: 7.81-500 pg/mL
Recovery Range: 80-102%
Save Method: 2-8℃
Standard graph:
Background: Interleukin 33 (IL-33) Also known as NF-HEV And DVS 27 , yes IL-1 Family members, with a molecular weight of 30 kDa , by more than Various types of cell secretion, including fibroblasts, mast cells, dendritic cells, macrophages, osteoblasts, endothelial cells and Epithelial cells. IL-33 Inducible adjuvant T Cells, mast cells, eosinophils, and basophils secretion II Type cell Factor. IL-33 By interacting with the receptor ST2 And IL-1 Receptor accessory protein (IL1RAP) Binding, activation can promote polarization Th2 Cells secretion II Type cytokine ( Such as IL-5 And IL-13) Of NF-κB And MAP Extracellular molecules in the kinase signaling pathway to mediate their biological Learning function. Injection IL-33 After, it can be induced in vivo II Type cytokines, which are thought to induce severe pathological changes in mucosal organs. |
| Storage Temp. | Stored at 2 ~ 8 ℃, the validity period is two years. |

