Standard curve
Example of IL-13 standard curve in Assay Diluent .
Mouse IL-13 Single Plex Assay (for Luminex)
Mouse IL-13 Single Plex Assay (for Luminex)
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Product Details
Product Details
Product Specification
| Antigen | IL-13 |
| Reactivity | Mouse |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8°C as supplied. |
Kit
| Precision | Intra-assay: ＜10%； Inter-assay: ＜20% |
| Sample type | Serum; Plasma; Cell culture supernatant |
| Assay type | Sandwich (quantitative) |
| Sensitivity | ＜2pg/mL |
| Range | 0.26pg/mL-50000pg/mL |
| Recovery | Plasma: 80%-120% Serum: 80%-120% Cell culture supernatant: 80%-120% |
| Assay time | 70 minutes |
| Species reactivity | Mouse |
Background
Interleukin 13 (IL-13) is a protein that in humans is encoded by the IL13 gene. The secondary structural features of IL-13 are similar to that of Interleukin 4 (IL-4); however it only has 25% sequence identity to IL-4 and is capable of IL-4 independent signaling. IL-13 is a cytokine secreted by T helper type 2 (Th2) cells, CD4 cells, natural killer T cell, mast cells, basophils, eosinophils and nuocytes. Interleukin-13 is a central regulator in IgE synthesis, goblet cell hyperplasia, mucus hypersecretion, airway hyperresponsiveness, fibrosis and chitinase up-regulation. It is a mediator of allergic inflammation and different diseases including asthma.
Picture
Picture
Luminex
Quantifiction of Mouse spleen cells
Stimulated sample: Mouse spleen cells were stimulated with 4 ug/mL ConA for 24h, and the average concentration of IL-13 in the supernatant was measured to be 211.99 pg/mL.
Unstimulated sample: Mouse spleen cells were cultured for 24h, and the average concentration of IL-13 in the supernatant was measured to be 1.35 pg/mL.
Recovery
Within the detection range, the combined cytokine proteins were added to serum,plasma at high、low concentrations.The matrices used in these experiments were diluted 2-fold with the assay buffer before the addition of the cytokine proteins. The results were compared with those of samples that had the same concentration of cytokines added in the assay buffer.
Protocol Diagram
