V5 Tag: A Versatile Protein Labeling System Empowering Modern Molecular Biology

23 Apr 2026 by AntBio
V5 Tag: A Versatile Protein Labeling System Empowering Modern Molecular Biology

V5 Tag: A Versatile Protein Labeling System Empowering Modern Molecular Biology


Published by ANT BIO PTE. LTD.

Concept


The V5 tag is a 14‑amino‑acid linear peptide epitope (GKPIPNPLLGLDST) derived from the P‑kappa mutant of the simian virus 40 (SV40) small t antigen. At just ~1.5 kDa, this compact, highly immunogenic tag enables robust detection and purification of target proteins across prokaryotic and eukaryotic systems. Its balanced hydrophilicity and linear epitope nature ensure reliable recognition by specific antibodies under both native and denaturing conditions, while imposing minimal structural interference on fusion proteins. These properties establish the V5 tag as a cornerstone tool for protein expression, localization, interaction, and purification studies.

[Image Location: V5 Tag Structure, Sequence, and Antigen Recognition Diagram]

Research Frontier


The V5 tagging system has evolved from a basic detection tool to a multifunctional platform integrated with cutting‑edge technologies including CRISPR‑mediated endogenous labeling, super‑resolution imaging, single‑cell proteomics, and proximity‑dependent biotinylation. Innovations such as tandem V5 repeats, conditionally stabilized tags, and photoactivatable V5 probes have expanded its utility in dynamic protein tracking, interactome mapping, and functional genomics. When combined with high‑affinity V5 antibodies and optimized reagents, the V5 system enables sensitive, reproducible characterization of proteins in physiologically relevant contexts.

Research Significance


Accurate protein analysis demands minimal‑interference labeling tools. The V5 tag addresses key limitations of larger fusion partners (e.g., GFP) and context‑dependent epitopes by providing consistent, high‑affinity recognition without disrupting protein folding, trafficking, or function. It supports rigorous investigation of protein expression, subcellular localization, complex assembly, post‑translational modification, and degradation. By enabling precise manipulation and monitoring of proteins—from basic research to therapeutic development—the V5 system accelerates discovery in cell biology, proteomics, gene editing, and drug development.

Related Mechanisms and Research Applications


Core Features of the V5 Tag


  • Small size (~1.5 kDa) minimizes functional disruption
  • Linear epitope enables detection under reducing/denaturing conditions
  • High immunogenicity supports sensitive detection (pg‑level sensitivity)
  • N‑ or C‑terminal fusion flexibility for versatile design
  • Compatible with all major expression systems (bacterial, yeast, insect, mammalian)

Key Research Applications


  1. Protein Detection

    Western Blot, immunofluorescence (IF), immunohistochemistry (IHC), flow cytometry
  2. Protein Interaction

    Immunoprecipitation (IP), Co‑IP, affinity purification‑mass spectrometry (AP‑MS)
  3. Protein Purification

    Gentle immunoaffinity purification under native conditions
  4. Functional Genomics

    CRISPR knock‑in labeling, ORF library screening, protein localization profiling
  5. Advanced Technologies

    Proximity labeling, super‑resolution imaging, single‑cell proteomics

ANTBIO Product Empowerment


ANT BIO PTE. LTD. offers a fully optimized V5 tagging platform to support reliable protein research:

  • Anti‑V5 Tag Antibodies (Starter brand)

    High‑affinity monoclonal and polyclonal antibodies validated for WB, IP, IF, IHC, and flow cytometry with ultra‑low background.
  • V5 Tag Protein Purification Resin (Absin brand)

    Agarose‑conjugated anti‑V5 antibody for high‑specificity native purification of V5‑fusion proteins.
  • V5 Tag Peptide (Absin brand)

    Competitor elution peptide for gentle, reversible protein recovery.
  • General Labeling & Detection Reagents (Absin brand)

    Optimized buffers, blockers, and diluents for consistent V5‑based assays.

This integrated toolkit ensures robust, reproducible results across every stage of protein research.

Brand Mission


ANT BIO PTE. LTD. is committed to advancing life science research by providing high‑quality, reliable protein research tools for labeling, detection, purification, and functional analysis. Our comprehensive product portfolio — including tag antibodies, recombinant proteins, assay kits, and universal reagents — empowers scientists worldwide to achieve consistent, impactful results. We strive to be the trusted partner that accelerates discovery in cell biology, proteomics, gene editing, and translational medicine.

Related Product List


  1. Anti-V5 Tag Monoclonal Antibody

    Catalog No.: S0Bxxxx

    Applications: WB, IP, IF, IHC, Flow Cytometry

    Reactivity: Recombinant V5‑fusion proteins

    Features: High sensitivity, low background, lot‑to‑lot consistency

  2. Anti-V5 Tag Polyclonal Antibody

    Catalog No.: S0Bxxxx

    Applications: WB, IP, ELISA

    Host: Rabbit

  3. V5-Tag Agarose Resin

    Catalog No.: absxxxx

    For: Immunoaffinity purification of V5‑fusion proteins

  4. V5 Tag Competitive Elution Peptide

    Catalog No.: absxxxx

    For: Gentle native elution in purification

  5. Protein Tag Detection Buffer Kit

    Brand: Absin

    For: Optimized WB/IF/IHC performance



ANT BIO PTE. LTD. – Empowering Scientific Breakthroughs

At ANTBIO, we are committed to advancing life science research through high-quality, reliable reagents and comprehensive solutions. Our specialized sub-brands (Absin, Starter, UA) cover a full spectrum of research needs, from general reagents and kits to antibodies and recombinant proteins. With a focus on innovation, quality, and customer-centricity, we strive to be your trusted partner in unlocking scientific mysteries and driving medical progress. Explore our product portfolio today and elevate your research to new heights.
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