Triple Breakthrough in IFN-γ ELISA Kits: Higher Sensitivity, Faster Speed, and Broader Linearity

Triple Breakthrough in IFN-γ ELISA Kits: Higher Sensitivity, Faster Speed, and Broader Linearity

 

Interferon - γ (IFN - γ) is the sole member of the type II interferon family. It is a pro - inflammatory cytokine mainly secreted by activated T cells (such as Th1, CTL) and natural killer (NK) cells. Its molecular structure is a biologically active homodimer. It activates the JAK - STAT signaling pathway by binding to IFNGR1/IFNGR2 receptors to regulate the expression of downstream genes. As a core regulator of the immune system, IFN - γ has a broad - spectrum antiviral function. It inhibits viral replication by inducing interferon - stimulated genes such as Mx protein and protein kinase R, and enhances the expression of MHC - I/II molecules to promote antigen presentation. In immune regulation, it drives Th1 - type immune responses, activates the tumor - killing function of macrophages, and at the same time recruits immune cells to the tumor microenvironment through the secretion of CXCL9/10/11 chemokines to play an anti - tumor role. Clinical detection of IFN - γ is of great value for the diagnosis of infectious diseases and the evaluation of the efficacy of tumor immunotherapy.

 

Innovative Advancement! Starter Bio proudly introduces three groundbreaking one-step IFN-γ ELISA kits, meticulously designed to meet diverse experimental needs with unparalleled precision.

Human IFN - γ OneStep ELISA Kit: Hypersensitive Residual Detection

With a sensitivity of 3.4 pg/ml and a detection range of 3.4 - 217 pg/ml, it has been calibrated and verified by the NIBSC international standard. It can precisely capture trace samples, making it the first choice for residual detection! The experiment only takes 1 hour!

 

Human IFN - γ Broad range ELISA Kit: Wide - range Coverage

The linear range is 0.8 - 1739 pg/ml. One kit can handle samples with widely different concentrations, eliminating the trouble of dilution!

 

Human IFN - γ OneStep OptiQuant ELISA Kit: OptiQuant Range Competing with Big - name Products, Faster, More Accurate

The detection range is 15 - 1000 pg/ml. The experiment can be completed in 45 minutes, enabling efficient and rapid detection without waiting!

 

Hypersensitive Residual Detection Kit (Only 1h Required)

Human IFN - γ OneStep ELISA Kit

Catalog Number: S0C3005

Sensitivity: 0.6 pg/ml

Range: 3.4 pg/ml – 217.4 pg/ml

Assay time: 60 minutes

Precision: Intra - assay: 5.0%; Inter - assay: 6.2%

Recovery: Cell culture supernatant 105%; Serum 83%; Plasma 84%

Standard Curve

 

 

Calibration with International Standard

 

 

Example of the standard curve of NIBSC (87/586) (using the kit diluent #1).

The human interferon - γ standard has been calibrated by NIBSC (87/586), and the calibration factor (CF) is 1.0.

 

Wide - range Coverage Kit (Only 45min Required)

Human IFN - γ Broad range ELISA Kit

Catalog Number: S0C3025

Sensitivity: 0.56 pg/ml

Range: 0.80 pg/mL – 1739.20 pg/mL

Assay time: 45 minutes

Precision: Intra - assay: 5.1%; Inter - assay: 9.2%

Recovery: Cell culture supernatant 98%; Serum 89%; Plasm 87%

Standard Curve

 

 

OptiQuant Kit (Only 45min Required)

Human IFN - γ OneStep OptiQuant ELISA Kit

Catalog Number: S0C3040

Sensitivity: 2.577 pg/ml

Range: 15.63 pg/mL – 1000 pg/m

Assay time: 45 minutes

Precision: Intra - assay: 4.0%; Inter - assay: 4.5%

Recovery: Cell culture supernatant: 93%; Serum: 109%; Plasma: 111%

Standard Curve

 

 

Spike - in Linearity

 

 

To verify the linear range of the detection method, three samples (including different matrices) were spiked with high - concentration human interferon - γ. After gradient dilution with the supporting calibration diluent, the sample values all fell within the detection range.

 

For more one - step ELISA kits, click on the product catalog numbers below to access detailed information on our official website.

 

Product Information

Catalog Number Product Name Price
S0C3015 Mouse Ig isotype OneStep ELISA Kit $535 
S0C3035 Mouse IFN-γ OneStep ELISA Kit $535 
S0C3030 Mouse IL-10 OneStep ELISA Kit $535 
S0C3029 Mouse IL-1β OneStep ELISA Kit $535 
S0C3023 Mouse TNF-α OneStep ELISA Kit $535 
S0C3020 Mouse IL-2 OneStep ELISA Kit $535 
S0C3019 Mouse IL-6 OneStep ELISA Kit $535 
S0C3028 Mouse IgA OneStep ELISA Kit $535 
S0C3022 Mouse IgM OneStep ELISA Kit $535 
S0C3034 Mouse IgA Competitive ELISA Kit (broad range) $535 
S0C3038 Mouse IgG (Total) Broad range ELISA Kit $535 
S0C3002 Rabbit IgG OneStep ELISA Kit $470 
S0C3039 Rat IgA OneStep ELISA Kit $535 
S0C3012 Rat IgG OneStep ELISA Kit $470 
S0C3027 Rat IgM OneStep ELISA Kit $535 
S0C3037 Rat IgE OneStep ELISA Kit $535 
S0C3016 His tag OneStep ELISA Kit $470 
S0C3017 His tag Competitive ELISA Kit (broad range) Inquiry
S0C3031 E. coli Host Cell Proteins OneStep ELISA Kit $670 
S0C3033 M13 OneStep ELISA Kit $535 
S0C3032 Protein A OneStep ELISA Kit $520 
S0C3044 Human Serum Albumin Competitive OneStep ELISA Kit $535 
S0C3040 Human IFN-γ OneStep OptiQuant ELISA Kit $535 
S0C3021 Human Serum Albumin OneStep ELISA Kit $535 
S0C3007 Human IgG (Total) OneStep ELISA Kit $470 
S0C3041 Human Granzyme B OneStep ELISA Kit $535 
S0C3011 Human GM-CSF OneStep ELISA Kit $470 
S0C3018 Human IFN-α OneStep ELISA Kit $535 
S0C3005 Human IFN-γ OneStep ELISA Kit $470 
S0C3014 Human IL-15 OneStep ELISA Kit $470 
S0C3013 Human IL-1β OneStep ELISA Kit $2,670 
S0C3024 Human TNF-α OneStep ELISA Kit $470 
S0C3001 Human IL-2 OneStep ELISA Kit $470 
S0C3009 Human IL-5 OneStep ELISA Kit $470 
S0C3004 Human IL-6 OneStep ELISA Kit $470 
S0C3036 Human IL-8 OneStep ELISA Kit $535 
S0C3043 Human IgA OneStep ELISA Kit $535 
S0C3042 Human IgE OneStep ELISA Kit $535 
S0C3026 Human IgM OneStep ELISA Kit $535 
S0C3010 Human ST2 OneStep ELISA kit $470 
S0C3025 Human IFN-γ Broad range ELISA Kit $535 
S0C3003 Human IL-13 OneStep ELISA Kit $470 
S0C3008 Mouse IgG (Total) OneStep ELISA Kit $470