The Essential Role of CK-Pan Antibody in Cell Biology and Tumor Research
The Essential Role of CK-Pan Antibody in Cell Biology and Tumor Research
Concept
Cytokeratins (CK) are major intermediate filament proteins that form the cytoskeleton of epithelial cells. Pan-Cytokeratin (CK-Pan) antibodies are broad-spectrum reagents that recognize conserved epitopes across multiple type I and type II cytokeratins, making them universal markers for identifying epithelial lineage cells, tracing tumor origin, and assessing tissue differentiation.
Research Frontier
CK-Pan antibodies are widely used in modern cell biology, pathology, and liquid biopsy. Current advances focus on fluorescent-conjugated formats, multiplex imaging, CTC detection, digital pathology, and AI-assisted quantification, enabling high-precision epithelial cell tracing and minimal residual disease monitoring.
Research Significance
CK-Pan antibodies are irreplaceable tools because they:
- Identify epithelial cells and distinguish epithelial vs. mesenchymal lineages
- Diagnose and subtype epithelial-derived carcinomas
- Detect micrometastases and circulating tumor cells (CTCs)
- Monitor epithelial-mesenchymal transition (EMT)
- Support tissue engineering and regenerative medicine quality control
Core Mechanisms and Technical Applications
Biological Function of Cytokeratins
- Form 10 nm intermediate filaments to maintain cell structure and mechanical stability
- Regulate intracellular signaling, mechanotransduction, and cell polarity
- Strictly expressed in epithelial cells; absent in mesenchymal, hematopoietic, and nervous tissue
Value of CK-Pan Broad-Spectrum Recognition
- Detects CK1–CK8, CK10, CK14–CK16, CK19, and other subtypes
- Ensures high sensitivity in heterogeneous tissues and tumors
- Enables reliable identification even in poorly differentiated carcinomas
Applications in Tumor Pathology
- Labels >95% of carcinomas to confirm epithelial origin
- Distinguishes carcinomas from sarcomas and lymphomas
- Detects minimal tumor deposits with sensitivity down to 0.01%
- Supports staging, prognostic evaluation, and metastasis tracking
Technical Optimization
- Recommended antigen retrieval: citrate buffer (pH 6.0), heat-induced
- IHC dilution range: 1:50 to 1:200
- Positive signal: cytoplasmic filamentous staining
- Requires positive and negative controls for validation
Emerging Applications
- Circulating tumor cell (CTC) enrichment and detection
- Multicolor immunofluorescence and flow cytometry panels
- Tissue-engineered epithelium maturation assessment
- Tumor heterogeneity and single-cell profiling
Product Empowerment
ANT BIO PTE. LTD. provides the S-RMab® Pan-Cytokeratin Recombinant Rabbit Monoclonal Antibody, Alexa Fluor® 488 Conjugated (S-P001, Cat. No. S0B0188) under its Starter product line.
This fluorescent-conjugated antibody delivers bright, stable green fluorescence and broad cytokeratin recognition, ideal for high-performance flow cytometry, immunofluorescence imaging, CTC detection, and multicolor panel studies.
Core Product Advantages
|
Core Product Advantages |
|
Superior Fluorescence Performance: Alexa Fluor® 488 conjugate delivers high brightness, photostability, and compatibility with flow cytometers and confocal microscopes. |
|
Broad-Spectrum CK Recognition: Detects multiple cytokeratin subtypes for universal epithelial cell labeling with high specificity and low background. |
|
Excellent Stability & Batch Consistency: Rigorous QC ensures minimal inter-batch variation and reliable performance in clinical and research applications. |
|
Broad Sample Compatibility: Suitable for cell suspensions, FFPE sections, frozen tissues, and smears. |
Key Application Scenarios
|
Key Application Scenarios |
|
Epithelial Cell Identification & Sorting: Precise labeling and isolation of epithelial cells from mixed populations. |
|
Circulating Tumor Cell (CTC) Detection: Core reagent for enriching and detecting epithelial-derived CTCs from peripheral blood. |
|
Tumor Diagnosis & Subtyping: Determines epithelial origin in primary and metastatic cancers. |
|
Multicolor Flow & Imaging Panels: Ideal for immunophenotyping, tumor microenvironment analysis, and cell differentiation studies. |
|
Tissue Engineering & Regenerative Medicine: Evaluates epithelial maturation in engineered skin, cornea, and mucosal grafts. |
Brand Mission
ANT BIO PTE. LTD. is a leading provider of life science reagents, offering antibodies, recombinant proteins, assay kits, and general laboratory reagents. We operate three specialized sub-brands: Absin (general reagents & kits), Starter (antibodies), and UA (recombinant proteins), delivering integrated solutions for cell biology, oncology, pathology, and regenerative medicine. We are committed to supplying high-quality, consistent, and innovative tools to advance scientific discovery and clinical diagnostics.
Related Product List
|
Product Name |
Catalog No. |
Applications |
|
S-RMab® CK-Pan Recombinant Rabbit mAb (Alexa Fluor® 488) |
S0B0188 |
Flow cytometry, IF, CTC detection, multicolor imaging |
|
Unconjugated Pan-Cytokeratin Antibody |
Starter series |
IHC, WB, epithelial tumor diagnosis |
|
Fluorescent Conjugation Kits |
Absin series |
Custom antibody labeling |
|
Antigen Retrieval Buffers |
Absin series |
IHC sample preparation |
Brand Promotion Copy
ANT BIO PTE. LTD. – Empowering Scientific Breakthroughs
At ANTBIO, we are committed to advancing life science research through high-quality, reliable reagents and comprehensive solutions. Our specialized sub-brands (Absin, Starter, UA) cover a full spectrum of research needs, from general reagents and kits to antibodies and recombinant proteins. With a focus on innovation, quality, and customer-centricity, we strive to be your trusted partner in unlocking scientific mysteries and driving medical progress. Explore our product portfolio today and elevate your research to new heights
