CD44 Antibody – Analyzing Its Multifaceted Regulatory Roles in Tumor Progression and Cell Migration
CD44 Antibody – Analyzing Its Multifaceted Regulatory Roles in Tumor Progression and Cell Migration
Concept
CD44 is a broadly expressed transmembrane glycoprotein encoded by the CD44 gene on human chromosome 11. As a major cell adhesion receptor, it mediates cell–matrix interactions, migration, and signaling. Through extensive alternative splicing, CD44 exists as standard (CD44s) and variant isoforms (CD44v). CD44 is a well-established marker for cancer stem cells and a key regulator of tumor invasion, metastasis, and therapy resistance.
Research Frontier
CD44 research has shifted toward isoform-specific functions, proteolytic cleavage, intracellular domain signaling, and cancer stem cell (CSC) biology. Current advances focus on isoform-specific antibodies, spatial detection, and targeted therapeutic development to block migration, invasion, and metastatic progression.
Research Significance
CD44 is a central hub in tumor progression:
- Drives cell migration, invasion, and metastasis
- Serves as a defining marker for tumor stem cells
- Modulates drug resistance and disease recurrence
- Predicts poor prognosis in numerous solid tumors
- Enables precision subtyping and targeted intervention
Core Mechanisms and Research Methodology
Structural and Genetic Features
- Encoded on chromosome 11 with extensive alternative splicing
- Standard CD44 (CD44s) is ubiquitous; variant isoforms (CD44v) link to malignancy
- Extracellular domain binds hyaluronic acid, osteopontin, collagen, and other ligands
Key Roles in Tumor Progression
- Migration & Invasion: Promotes adhesion to extracellular matrix and enhances motility
- Cancer Stem Cell Phenotype: Maintains stemness, self-renewal, and therapy resistance
- Signal Transduction: Intracellular domain translocates to the nucleus to regulate metastatic gene expression
CD44 Isoform Functional Diversity
- CD44s: widely expressed, supports basic adhesion and migration
- CD44v: associated with advanced disease, metastasis, and poor survival
- Isoform expression profiles are tumor-type specific and clinically prognostic
Proteolytic Processing
- Sequential cleavage by metalloproteinases and γ-secretase
- Releases soluble ectodomain and intracellular domain (ICD)
- ICD translocates to the nucleus to drive survival and metastasis
Clinical Applications
- Serum soluble CD44 as a prognostic biomarker
- Tissue CD44 expression for tumor subtyping and risk stratification
- Targeted antibody and CAR-T therapy development
- Cancer stem cell detection and isolation
Product Empowerment
ANT BIO PTE. LTD. provides the CD44 Recombinant Rabbit Monoclonal Antibody (SDT-R107, Cat. No. S0B2228) under its Starter product line. This high‑specificity, high‑sensitivity reagent enables reliable detection of CD44 in FFPE tissues and cell samples, supporting cancer stem cell research, tumor staging, and migration studies.
Core Product Advantages
|
Core Product Advantages |
|
High Specificity & Precise Membrane Localization: Specifically recognizes human CD44; clear, distinct membrane staining in FFPE sections with clean background and high signal specificity. |
|
Excellent Stability & Batch-to-Batch Consistency: Strict quality control ensures minimal inter-batch variation and long‑lasting performance for clinical pathology and translational research. |
|
Broad Application Compatibility: Validated for IHC and ideal for cancer stem cell identification, tumor invasion assessment, and prognostic evaluation. |
Key Application Scenarios
|
Key Application Scenarios |
|
Cancer Stem Cell Research: Identification and isolation of CD44+ stem‑like populations in breast, colorectal, pancreatic, and other solid tumors. |
|
Tumor Invasion & Metastasis Evaluation: Assessment of CD44 expression levels linked to invasive potential, metastatic risk, and patient prognosis. |
|
Cell Adhesion & Migration Assays: Study of CD44–ligand interactions and mechanistic roles in cell motility. |
|
Inflammation & Immune Regulation: Analysis of CD44 functions in lymphocyte activation, homing, and tissue inflammation. |
Brand Mission
ANT BIO PTE. LTD. is a leading provider of life science reagents, offering antibodies, recombinant proteins, assay kits, and general laboratory reagents. We operate three specialized sub‑brands: Absin (general reagents & kits), Starter (antibodies), and UA (recombinant proteins), delivering integrated solutions for oncology, cancer stem cell biology, tumor metastasis, and precision medicine. We are committed to supplying high‑quality, consistent, and innovative tools to accelerate mechanistic research and therapeutic development.
Related Product List
|
Product Name |
Catalog No. |
Applications |
|
CD44 Recombinant Rabbit Monoclonal Antibody (SDT-R107) |
S0B2228 |
IHC, cancer stem cell detection, tumor profiling |
|
CD44 Variant Isoform Antibody Panel |
Starter series |
Isoform‑specific tumor subtyping |
|
IHC Detection Kits & Buffers |
Absin series |
Standardized staining and signal enhancement |
|
Recombinant CD44 Protein |
UA-series |
Antigen validation and ligand binding assays |
Brand Promotion Copy
ANT BIO PTE. LTD. – Empowering Scientific Breakthroughs
At ANTBIO, we are committed to advancing life science research through high-quality, reliable reagents and comprehensive solutions. Our specialized sub-brands (Absin, Starter, UA) cover a full spectrum of research needs, from general reagents and kits to antibodies and recombinant proteins. With a focus on innovation, quality, and customer-centricity, we strive to be your trusted partner in unlocking scientific mysteries and driving medical progress. Explore our product portfolio today and elevate your research to new heights.
