Selective Chemical Labeling of Protein Lysine Methacrylation

1. Literature Information

This work reveals a photocatalytic thiol–Michael addition strategy for highly specific chemical labeling and proteomic identification of lysine methacrylation, a newly discovered acylation modification highly similar to crotonylation.

2. Research Background

Lysine methacrylation is a structural isomer of crotonylation, widely distributed in histones and related to mitochondrial metabolism and genetic diseases. Traditional antibody tools cannot distinguish methacrylation from crotonylation due to high structural similarity, causing severe crossinterference and hindering functional study. There is an urgent need for chemical biology methods to achieve selective labeling and enrichment.

3. Research Approach

Based on thermodynamic and steric differences between methacrylamide and crotonamide, researchers designed a watersoluble probe with benzene thiol reactive group and azide handle. Under photocatalysis, thiol radicals selectively add to methacrylamide double bonds with minimal reaction to crotonamide. Labeled proteins are biotinylated via click chemistry, enriched by streptavidin beads and identified by highresolution mass spectrometry.

4. Research Outcomes

· The probe achieves over 85% selectivity for methacrylation even under high crotonylation background.

· This workflow enables systematic identification of novel methacrylation sites on histones and nonhistone proteins.

· Multiple new substrates involved in chromatin regulation, transcription and cell cycle are discovered, expanding the methacrylation regulatory network.

· The method overcomes limitations of antibodybased enrichment and enables unbiased proteomewide profiling.

5. Product Empowerment

ANT BIO PTE. LTD. provides highly specific methacrylation antibodies for mechanistic and omics research.

Methacryllysine Recombinant Mouse mAb (S3455, Cat. No.: S0B6453)

· Superior specificity with minimal crossreaction to propionylation, butyrylation and other similar modifications.

· High affinity and batch consistency for stable WB, IF, IHC and IP performance.

· Mouse IgG isotype compatible with multiplex labeling using rabbit primary antibodies.

· Ideal for enrichment and omics analysis of methacrylated proteins.

6. Brand Mission

ANT BIO PTE. LTD. is a leading life science reagent provider offering antibodies, proteins, kits and general reagents. Our subbrands Absin (general reagents & kits), Starter (antibodies) and UA (recombinant proteins) cover full research scenarios. We aim to supply innovative, reliable tools to promote metabolicepigenetic research and disease target discovery.

7. Related Product List

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