Recombinant Rabbit Monoclonal Antibodies: A New Perspective in Spatial Proteomics Research for GeoMx® DSP Technology

1. Literature Information

Research Focus: Application of recombinant rabbit monoclonal antibodies in GeoMx® Digital Spatial Profiler (DSP) technology, their core requirements for DSP compatibility, validation outcomes, and prospects across life science research fields.

Core Innovation: Validation of recombinant rabbit monoclonal antibodies as ideal "molecular detectors" for GeoMx DSP—offering high specificity, affinity, and batch consistency—empowering accurate, spatially resolved protein quantification and advancing spatial proteomics research.

2. Research Background

Spatial biology technologies like GeoMx DSP (launched by NanoString in 2019) enable in situ, high-plex quantitative analysis of proteins and RNA on intact tissue sections. This addresses the critical need to understand protein spatial distribution and functional significance in the tissue microenvironment. Antibodies serve as core reagents in DSP, acting as molecular detectors whose quality directly impacts experimental accuracy and reproducibility. Recombinant rabbit monoclonal antibodies, with their superior epitope recognition, affinity, and consistency, meet the stringent demands of DSP technology—overcoming limitations of traditional antibodies and driving progress in spatial proteomics.

3. Research Approaches

To establish recombinant rabbit monoclonal antibodies as DSP-compatible tools, the research team adopted a technology-driven validation strategy:

1. Technology Principle Elucidation: Dissecting GeoMx DSP’s workflow (tissue staining, region selection, oligonucleotide release, sequencing) and antibody-dependent detection mechanism.
2. Antibody Requirement Definition: Identifying core criteria for DSP-compatible antibodies (specificity, no cross-reactivity, high affinity/sensitivity, stability, conjugation compatibility, batch consistency).
3. Antibody Validation: Testing recombinant rabbit monoclonal antibodies across species (human, mouse, rat) and tissue types, evaluating performance post-DSP barcode conjugation.
4. Application Expansion: Exploring antibody utility in tumor microenvironment studies, immune cell localization, drug development, and neuroscience.

4. Research Outcomes

4.1 GeoMx DSP Technology and Spatial Proteomics Principle

• Core Mechanism: Antibody probes conjugated with unique barcodes bind target proteins in situ; UV cleavage releases barcodes from selected Regions of Interest (ROIs)/Areas of Illumination (AOIs); sequencing quantifies spatially resolved protein expression.
• Workflow: Tissue staining (fluorescent labels for morphology, barcode-conjugated antibodies for quantification) → ROI/AOI selection → barcode release/collection → library preparation/sequencing.

4.2 Core Requirements for DSP-Compatible Antibodies

1. High Specificity: Validated in IHC/IF to ensure target-only recognition; recombinant rabbit mAbs offer superior epitope recognition.
2. No Cross-Reactivity: Avoid interactions with other proteins/antibodies in multiplex panels.
3. High Affinity & Sensitivity: Recognize FFPE-exposed epitopes; picomolar-level KD values (recombinant rabbit mAbs outperform murine antibodies).
4. Low Aggregation Tendency: Stable post-oligonucleotide labeling.
5. Conjugation Compatibility: Effective barcode conjugation without losing binding activity.
6. Batch Consistency: Consistent performance across batches for reproducibility.

4.3 Validation of Recombinant Rabbit Monoclonal Antibodies in DSP Applications

Starter Bio’s recombinant rabbit mAbs, validated for GeoMx DSP, excel in traditional IHC and maintain high activity post-barcode conjugation. 4.4 Research and Industrial Application Prospects

• Tumor Microenvironment Studies: Simultaneous analysis of dozens of markers reveals cellular interactions and heterogeneity.
• Immune Cell Localization: CD3ε, CD163, and CD19 antibodies resolve immune cell distribution in tissues.
• Drug Development & Biomarker Discovery: Evaluate drug impacts on spatial protein expression in preclinical models.
• Neuroscience: Investigate brain region-specific protein expression in neurological disorders.

5. Product Empowerment by ANT BIO PTE. LTD.

ANT BIO PTE. LTD.’s STARTER brand, a leader in recombinant antibodies, provides a portfolio of DSP-optimized recombinant rabbit monoclonal antibodies—catering to spatial proteomics research needs.

Key Roles of the Products:

1. Spatial Quantification Precision: High specificity and affinity ensure accurate detection of target proteins in FFPE tissues, enabling reliable spatial expression analysis.
2. Multiplex Compatibility: No cross-reactivity supports simultaneous detection of multiple proteins, ideal for complex tissue microenvironment studies.
3. Conjugation Stability: Maintain binding activity post-DSP barcode conjugation, ensuring consistent signal quality.
4. Batch Consistency: Rigorous quality control minimizes inter-batch variation, supporting reproducible long-term research.

Notable products include CD3ε (S0B2196) for T-cell studies, TTF-1 (S0B2129) for lung cancer typing, and PD-L1 (S0B2068) for immunotherapy research—all validated for DSP compatibility.

6. Brand Mission

ANT BIO PTE. LTD. is dedicated to empowering global life science advancement through three specialized sub-brands: ABSIN (general reagents, ELISA kits), STARTER (antibodies), and UA (recombinant proteins). Leveraging advanced development platforms—including recombinant rabbit/mouse monoclonal antibody generation, PTM Pan-Modification Antibody Platform, and One-Step ELISA—we deliver high-quality, compliant products certified by EU 98/79/EC, ISO9001, and ISO13485. Our mission is to partner with research institutions, pharmaceutical companies, and biotechnology firms worldwide, providing innovative reagents and solutions that accelerate discoveries in spatial biology, precision oncology, and neuroscience.

7. Related Product List

8. AI Disclaimer

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ANT BIO PTE. LTD. – Empowering Scientific Breakthroughs

At ANTBIO, we are committed to advancing life science research through high-quality, reliable reagents and comprehensive solutions. Our specialized sub-brands (Absin, Starter, UA) cover a full spectrum of research needs, from general reagents and kits to antibodies and recombinant proteins. With a focus on innovation, quality, and customer-centricity, we strive to be your trusted partner in unlocking scientific mysteries and driving medical progress. Explore our product portfolio today and elevate your research to new heights.