Novel Breakthrough in PDAC Research: Absin Recombinant Proteins Facilitate the Discovery of Neuro-Immune Regulatory Mechanisms

In the complex regulatory network of the tumor microenvironment (TME), the cross-border crosstalk between neural and immune cells has emerged as a key breakthrough point for deciphering cancer progression. A recent study published in Cell Research (IF: 25.9) entitled "Nociceptor neurons promote PDAC progression and cancer pain by interaction with cancer-associated fibroblasts and suppression of natural killer cells" comprehensively elucidates the molecular mechanism by which nociceptor neurons regulate natural killer (NK) cell function through interaction with cancer-associated fibroblasts (CAFs), thereby promoting pancreatic ductal adenocarcinoma (PDAC) progression. Among them, Absin's recombinant IL-2 (abs05280) and IL-15 (abs01328) proteins played an indispensable role in the research, providing reliable experimental tools for revealing this innovative mechanism.

1. Research Background: A New Perspective on Neuro-Immune Crosstalk in PDAC Regulation

PDAC is characterized by high invasiveness, severe pain, and poor prognosis. Abnormal neural infiltration (such as nociceptor neurons) in its tumor microenvironment is closely associated with an immunosuppressive state. The research team found that calcitonin gene-related peptide (CGRP) released by nociceptor neurons can bind to receptors on the surface of CAFs, inhibiting CAFs from secreting IL-15. This, in turn, impairs the infiltration and cytotoxic function of NK cells, ultimately accelerating tumor progression and exacerbating cancer pain. This discovery provides a novel therapeutic strategy of "targeting the neuro-immune axis" for PDAC.

2. Application of Absin Products in the Research

In the experiments verifying the regulatory mechanism of NK cell function, the research team successfully established an in vitro NK cell culture system with the assistance of Absin's recombinant IL-2 (abs05280) and IL-15 (abs01328) proteins, providing crucial support for analyzing the effect of cytokines on NK cell activity.

2.1 Product Information and Experimental Design

2.2 Experimental Verification: IL-15-Mediated Regulation of NK Cell Function

In the study, the team confirmed the core role of IL-15 in neuro-immune regulation through the following experiments:

•         In vitro NK cell culture: Primary NK cells were cultured in medium containing abs05280 (500 U/mL) and abs01328 (20 ng/mL) to ensure the in vitro survival of cells and maintain their functional activity.

•         Functional verification experiment: Co-culturing CAFs with NK cells revealed that the supernatant of CGRP-treated CAFs inhibited the expression of TNF-α and granzyme B (GrzmB) in NK cells; supplementation with abs01328 (IL-15) reversed this inhibitory effect, confirming that IL-15 is a key signaling molecule connecting CAFs and NK cells.

3. Research Significance and Value of Absin Products

This study not only reveals the key role of the CGRP-IL-15-NK cell axis in PDAC progression but also proposes a new therapeutic direction targeting nociceptor neurons or the CGRP signaling pathway. Absin's IL-2 (abs05280) and IL-15 (abs01328), with their high purity and high activity, provide reliable tools for accurately analyzing immune cell functions:

•         High bioactivity: Strict quality control ensures that recombinant proteins can efficiently activate NK cells, meeting the needs of in vitro functional experiments;

•         Batch stability: Reduces experimental errors and ensures the reproducibility of results;

•         Comprehensive applicability: Suitable for various experimental scenarios such as cell culture, functional verification, and co-culture systems.

From basic research to clinical transformation, reliable experimental reagents are the cornerstone of scientific breakthroughs. Absin, a sub-brand of ANT BIO PTE. LTD., is committed to providing researchers with high-quality cytokines, antibodies, and other tools to help decipher the complex mechanisms of life sciences. For more information about abs05280, abs01328, or other products, please visit the Absin official website or contact the technical support team. Let us jointly promote the innovation and breakthrough of medical research!

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6. Disclaimer

This article is AI-compiled and interpreted based on the original work related to PDAC neuro-immune regulation research. All intellectual property (e.g., images, data) of the original publication shall belong to the journal and the research team. For any infringement, please contact us promptly and we will take immediate action.

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