Multi - color Flow Cytometry Mini Panel for Macrophage Identification
11 Jun 2025
by AntBio
MDSCs are derived from bone marrow progenitor cells and immature myeloid cells (IMCs). Under normal conditions, they serve as precursors to dendritic cells, macrophages, and granulocytes. They can rapidly differentiate into mature granulocytes, DCs, and macrophages, which then migrate to relevant organs and tissues to perform normal immune functions. IMCs constitute approximately 0.5% of peripheral blood mononuclear cells. However, under pathological conditions such as tumors, infections, inflammation, sepsis, and surgical trauma, the maturation of these myeloid - derived precursor cells is blocked due to the action of cytokines. As a result, they remain at various differentiation stages and become MDSCs with immune - suppressive functions. Acting on cytokines, MDSCs are recruited, migrate, and expand. Their quantity and proportion in peripheral blood increase by about tenfold, accounting for roughly 10% of peripheral blood mononuclear cells in patients and spanning the entire disease - development process.
Panel Recommendations
| Marker | Fluorochrome | Max EX/EM (nm) | Corresponding Laser | Filter | Product Number | Format (1 T represents one test) |
| CD11b | PerCP - Cy5.5 | 482/676 | Blue Laser | 695/40 | S0B5160 | 20/50/100/500 T available |
| CD33 | Alexa Fluor 488 | 494/517 | Blue Laser | 530/30 | S0B1604 | 20/50/100/500 T available |
| HLA - DR | PE | 496, 566/576 | Blue or Yellow - Green Laser | 575/26 | S0B5039 | 20/50/100/500 T available |
| CD15 | PE - Cy7 | 496, 566/576 | Blue or Yellow - Green Laser | 780/60 | abs1840175 | 250/1000 T available |
| CD14 | APC | 651/660 | Red Laser | 660/20 | S0B5194 | 20/50/100/500 T available |
Notes:
Since PMN - MDSCs are difficult to distinguish from neutrophils, consider adding CD115. It's reported that murine PMN - MDSCs express higher levels of CD115 than neutrophils.
Detailed Explanation of Each Antibody Marker's Function:
| CD antigen | Cellular expression | Molecular weight (kDa) | Functions |
| CD11b | Myeloid and NK cells | 170 | αM subunit of integrin CR3 (associated with CD18): binds CD54, complement component iC3b, and extracellular matrix proteins |
| HLA-DR | antigen-presenting cells such as dendritic cells, macrophages, and B cells | α （34 ） β （ 29k） | presentation of peptides to CD4+ T lymphocytes |
| CD33 | Myeloid progenitor cells, monocytes | 67 | Binds sialoconjugates |
| CD14 | Myelomonocytic cells | 53–55 | Receptor for complex of lipopolysaccharide and lipopolysaccharide binding protein (LBP) |
| CD15 | Neutrophils, eosinophils, monocytes | 180-220 | Terminal trisaccharide expressed on glycolipids and many cell-surface glycoproteins |
