Crotonylation Reshapes Histone Modification and Transcription

1. Concept

Lysine crotonylation is a conserved histone acylation modification identified in 2011. It carries an unsaturated crotonyl group, structurally distinct from acetylation, and strongly promotes gene transcription.

2. Research Frontier

Crotonylation is a major transcriptionactivating mark enriched at active promoters and enhancers. p300 acts as both crotonyltransferase and acetyltransferase. SIRT1–3 exhibit decrotonylase activity. CrotonylCoA level links cellular metabolism to chromatin state.

3. Research Significance

· Crotonylation more effectively relaxes chromatin than acetylation due to larger steric size and hydrophobicity.

· It is specifically recognized by YEATS domain proteins, recruiting transcription machinery.

· It regulates inflammatory response, cell differentiation and germ cell development.

· Dysregulation contributes to cancer, neurological disorders and metabolic diseases.

4. Mechanism and Application

· Regulation: p300 mediates crotonylation; SIRTs remove crotonyl groups.

· Function: Neutralizes histone charge, loosens chromatin, enhances transcription.

· Methods: WB, IF, IHC, IP, ChIPseq, modification mass spectrometry.

· Product application: Pancrotonylation antibodies enable global and sitespecific detection.

5. Brand Mission

ANT BIO PTE. LTD. is a premium life science reagent provider offering antibodies, proteins, kits and general reagents. Our subbrands Absin (general reagents & kits), Starter (antibodies) and UA (recombinant proteins) support epigenetic research, transcriptional regulation and disease target discovery.

6. Related Product List

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