Collaboration Between ANT BIO PTE. LTD. and TissueGnostics: Multi-Dimensional Analysis of Tissue In Situ Multiplex Staining in Tumor Microenvironment

1. Tumor Microenvironment (TME)

The Tumor Microenvironment (TME), the internal environment where tumor cells generate and survive, is a complex integrated system. It not only includes cellular components such as tumor cells themselves and inflammatory/immune cells but also non-cellular components such as extracellular matrix (ECM) and cytokines. The positive response to immunotherapy usually depends on the interaction between tumor cells and immune regulation within the Tumor Microenvironment (TME). Under these interactions, TME plays an important role in inhibiting or enhancing immune responses. Understanding the interaction between immunotherapy and TME is not only the key to analyzing the mechanism of action but also of great significance for providing new methods to improve the efficacy of current immunotherapy

Key components of TME include Tumor-Associated Macrophages (TAM), T cells, Dendritic Cells (DC), Cancer-Associated Fibroblasts (CAF), tumor vasculature, extracellular matrix (ECM), monocytes, neutrophils, eosinophils, regulatory B cells, lymphatic vessels, endothelial cells, pericytes, adipocytes, neurons, natural killer cells, suppressor cells, etc. These components interact with each other to form a complex network that regulates tumor progression, invasion, and metastasis.

2. Advantages and Applications of Multiplex Immunohistochemistry (mIHC) Technology

Multiplex immunohistochemistry (mIHC) technology has unique advantages in evaluating the expression abundance, interaction, and spatial phenotypic information of various cellular components in the TME. It can simultaneously label multiple targets on the same section, helping researchers quantitatively evaluate cell phenotypes and activity, comprehensively depict the tumor immune microenvironment and in-situ spatial information between cells. This enables in-depth understanding of tumorigenesis mechanisms and is more likely to predict tumor response to treatment

In this solution, we used TissueFAXS Cytometry panoramic tissue flow cytometry quantitative analysis technology from TissueGnostics combined with Multiplex Fluorescence IHC Staining Kits from the Absin product line of ANT BIO PTE. LTD. to carry out multi-color immunohistochemistry application exploration in relevant samples.

3. Non-Specific Staining Image Processing

Figure 1. Original images and processed images of non-specific staining. The processed images effectively eliminate non-specific staining, ensuring the accuracy and reliability of subsequent analysis (Provided by TissueGnostics).

4. Multi-Color Spatial Phenotypic Analysis of Tissues in Tumor Microenvironment

Figure 2. Multi-color spatial phenotypic analysis of tumor microenvironment. The combination of multiplex fluorescence staining and TissueFAXS Cytometry technology realizes the precise analysis of cell populations in the tumor microenvironment and their spatial distribution (Provided by TissueGnostics).

5. Online Webinar Announcement

In view of the combined application of the above two technologies, ANT BIO PTE. LTD. and TissueGnostics will hold an online webinar themed "Multi-Dimensional Analysis of Tissue In Situ Multiplex Staining in Tumor Microenvironment" from 10:00 to 12:00 on May 19th. There are also multi-color kits and secondary antibody kits with special preferential prices!

Webinar Details:

•         Theme: Multi-Dimensional Analysis of Tissue In Situ Multiplex Staining in Tumor Microenvironment

•         Time: 10:00-12:00, Thursday, May 19th

•         Speakers:
       

￮       Product Manager of Absin (Sub-brand of ANT BIO PTE. LTD.): Tumor Microenvironment Research Tool - Multiplex Staining Techniques from Examples

￮       Dr. Xiaodong Gong, Senior Application Expert of TissueGnostics: Multi-Target Tissue Multi-Color and Spatial Phenotypic Microenvironment Analysis

•         Agenda:
       

￮       Session 1 (Absin Product Manager): 1. Advantages and Principles of Multiplex Fluorescence Staining; 2. Staining Operations and Techniques from Examples; 3. Multi-Color Related Reagents and Services

￮       Session 2 (Dr. Xiaodong Gong): 1. Tissue In Situ Single-Cell Spatial Phenotypic Analysis; 2. Heterogeneous Microenvironment Cell Population Interaction Analysis; 3. Integration of Multi-Color Immunofluorescence with Multi-Omics: Spatial Bioinformatics Data Solutions

•         Technical Support: TissueGnostics

•         Registration: Free registration and appointment are available

6. About ANT BIO PTE. LTD.

ANT BIO PTE. LTD. is a high-tech enterprise engaged in biological reagents and consumables. Adhering to the development philosophy of "Creating Convenience for Life Science! Creating Value for Customers! Creating a Happy Life for Employees!", the company has a professional product R&D and quality inspection team, marketing team, and advanced warehousing and logistics management team. Its self-operated brand Absin® mainly operates products including: biochemical reagents (common biochemical reagents, common reagents for biological experiments, fluorescent dyes, agonists/inhibitors, plant extracts), cell biology products (cell culture products, serum, additives, detection kits, consumables), immunology products, molecular biology products, product customization and detection services.

7. About TissueGnostics

TissueGnostics (TG), an Austrian company, is committed to developing overall solutions for tissue in situ single-cell image quantitative analysis, and researching tools that can serve researchers, assist clinical diagnosis of difficult and miscellaneous diseases, and screen tissue in situ drug targets. Since its establishment, TissueGnostics has established extensive and in-depth cooperation with major European scientific research institutes and enterprises, continuously solving problems for front-line researchers, and has accumulated rich experience in tissue image quantification technology. Its TissueFAXS Cytometry technology provides users with various solutions from imaging to analysis in the industry, solving the pain points of difficult accurate quantification and low credibility of image data, especially tissue images.

8. Featured Product: Absin Multiplex Fluorescence IHC Staining Kits

The Absin Multiplex Fluorescence IHC Staining Kits from ANT BIO PTE. LTD. can achieve multi-labeling on a single slide. Whether it is observing the tumor microenvironment, immune infiltration, performing cell counting or co-localization analysis, it can be realized. Currently, the maximum can reach six indicators and seven colors~

9. Related Product List

10. References

1. Tang, H., J. Qiao, and Y.X. Fu, Immunotherapy and tumor microenvironment. Cancer Lett, 2016. 370(1): p. 85-90.
2. Tsujikawa, T., et al., Quantitative Multiplex Immunohistochemistry Reveals Myeloid-Inflamed Tumor-Immune Complexity Associated with Poor Prognosis. Cell Rep, 2017. 19(1): p. 203-217.
3. Chen, S., et al., Multiomic Analysis Reveals Comprehensive Tumor Heterogeneity and Distinct Immune Subtypes in Multifocal Intrahepatic Cholangiocarcinoma. Clin Cancer Res, 2022. 28(9): p. 1896-1910.
4. Chen, X., et al., CD8(+) T effector and immune checkpoint signatures predict prognosis and responsiveness to immunotherapy in bladder cancer. Oncogene, 2021. 40(43): p. 6223-6234.
5. Wang, T., et al., Spatial distribution and functional analysis define the action pathway of Tim-3/Tim-3 ligands in tumor development. Mol Ther, 2022. 30(3): p. 1135-1148.

11. Disclaimer

This article is AI-compiled and interpreted based on the original work in document 1225.docx (Collaboration Between Absin and TG for Multi-Dimensional Analysis of Tissue In Situ Multiplex Staining in Tumor Microenvironment). All intellectual property (e.g., images, data) of the original publication shall belong to ANT BIO PTE. LTD., TissueGnostics and the relevant research team. For any infringement, please contact us promptly and we will take immediate action.

12. Brand Promotion Copy

ANT BIO PTE. LTD. – Empowering Scientific Breakthroughs

At ANTBIO, we are committed to advancing life science research through high-quality, reliable reagents and comprehensive solutions. Our specialized sub-brands (Absin, Starter, UA) cover a full spectrum of research needs, from general reagents and kits to antibodies and recombinant proteins. With a focus on innovation, quality, and customer-centricity, we strive to be your trusted partner in unlocking scientific mysteries and driving medical progress. Explore our product portfolio today and elevate your research to new heights.