Absin Bioluminescence Detection Solution — Comprehensive Product Portfolio, Benchmarked Against Imported Gold Standards

1. Cell Viability & Apoptosis Detection Kits

Abcam's CTG-LTM and CTF-LTM series products are based on the bioluminescence detection principle of luciferase and the detection principle of live-cell protease activity to measure cell viability. The CAS-GTM product utilizes the activity of Caspase 3/7 enzymes to design corresponding bioluminescent substrates for more sensitive detection of early cell apoptosis. The entire product line is fully comparable to the industry gold standard P* brand, with advantages such as simple operation, wide linear range, high sensitivity, stable signal, and suitability for high-throughput automated scenarios.

Fig. 1. Schematic diagram of the principle of cell viability detection using the luciferase method in the CTG-LTM series


Fig. 2. Schematic diagram of the principle of live-cell protease activity detection in the CTF-LTM series


Fig. 3. Schematic diagram of the principle of caspase 3/7 enzyme activity detection in the CAS-GTM series

2. Luciferase Reporter Gene Assay

Fig. 4. Schematic diagram of the detection results for the DUG-LTM Dual-Luciferase Reporter Assay Kit

Plant HEK293 cells at a confluence of 60% into a white 96-well plate with a clear bottom at a volume of 100 µl per well. On the second day, transfect the cells with 0.2 µg of DNA of two plasmids, pCMV-Fluc and pCMV-Rluc, per well. After 48 hours, measure the activity of the dual luciferases according to the product manual. The experimental data represent the average of four wells. Group A: The relative light units (RLU) after adding reagent component R1; Group B: The RLU after adding R2 buffer; Group C: The RLU after adding R2 substrate.

3. NanoLuc Luciferase Assay Reagents

Abcam's NAG-L™ NanoLuc luciferase product series, developed based on HiBiT technology (a bioluminescence detection technology based on protein complementation), can detect intracellular or extracellular protein expression, degradation, and interactions. These products are characterized by high sensitivity and a high signal-to-noise ratio.

Fig. 5. Schematic diagram of the lytic detection principle of NAG-L™ NanoLuc luciferase

4. Kinase Activity Assay Reagents

Abcam's KNG-L™ series of kinase activity assay reagents utilize ATP-dependent luciferase luminescence reactions to quantitatively measure the consumption of ATP during the kinase catalysis process, thereby assessing the catalytic activity of kinases. The ADP-K™ kinase ADP detection kit, on the other hand, can assess kinase catalytic activity by measuring the content of ADP.

Fig. 6. Schematic diagram of the detection principle of the ADP-K™ Kinase ADP Detection Kit

5. Other Luminescence Detection Reagents

Abcam also offers other detection reagents and consumables based on the principle of bioluminescence.