15-Minute Miracle: The Fastest Mouse Ig Isotyping ELISA Kit.

Immunoglobulin

Mouse immunoglobulins (Ig) play a critical role in immune responses and immune regulation. Based on the composition of heavy and light chains, they can be classified into IgG (IgG1, IgG2a, IgG2b, IgG3), IgM, IgA, and IgE. Depending on the antigenicity of the constant region of the light chain, the light chains of immunoglobulins can be divided into κ-type and λ-type. In mice, the ratio of κ chains to λ chains is approximately 20:1.

Applications of Isotyping Detection Kits

1. Hybridoma Screening: Hybridoma screening is an essential step in monoclonal antibody (mAb) development. Isotyping detection is performed at various stages, including hybridoma screening, mAb characterization, and post-purification validation, to accurately determine Ig subtypes and ensure the final monoclonal antibody has the correct subtype and functionality.

2. Animal Model Experiments: Immunoglobulin isotyping serves as an immune detection tool, aiding in monitoring and analyzing immune responses to pathogens and understanding their mechanisms in disease models.

3. Vaccine Development: Mouse immunoglobulin isotyping kits can be used to evaluate vaccine-induced immune responses. By detecting specific immunoglobulin subtypes (e.g., IgG, IgM, IgA) in mouse serum or plasma, researchers can assess the immunogenicity and efficacy of vaccines.

OneStep ELISA Kit

START introduces a new one-step ELISA kit for mouse Ig isotyping, requiring only 15 minutes to distinguish IgG1, IgG2a, IgG2b, IgG3, IgA, IgM, κ-type, and λ-type!

Experimental Principle

The kit employs a sandwich ELISA principle, with the plate pre-coated with tag antibodies for high-sensitivity capture detection. After adding samples and incubating with antibody pairs for 10 minutes, the plate is washed, and TMB substrate is added for color development for 3 minutes before stopping the reaction. The entire process takes only 15 minutes, offering rapid, simple, highly sensitive, and standardized detection, facilitating rapid qualitative analysis!

Kit Advantages

1. Fastest Mouse Ig Isotyping ELISA Kit on the Market: Requires only 15 minutes.

2. High-Sensitivity Tag Antibody Capture Technology: Ensures rapid detection without compromising accuracy.

3. Simple Operation: Eliminates the need for 3-4 hours of work; only one sample addition, one wash, and one incubation are required.

4. Low Matrix Effects and High Inter-Batch Consistency: Ensures reliable and reproducible results.

5. High Throughput: Enables simultaneous rapid detection of 10 independent samples.