Solution-WB
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2.1.2 Protein Ladder (Marker)
1.1.10 Membrane Proteins
7.7 p53
6.1 No Signal
Cause Investigation 1. Sample-related Causes(1) Sample does not express the target protein or has low expressionSelect an appropriate sample and conduct proper stimulation treatment.(2) Improper sample preparationAdd protease inhibitors and...
6.1 No Signal
Cause Investigation 1. Sample-related Causes(1) Sample does not express the target protein or has low expressionSelect an appropriate sample and conduct proper stimulation treatment.(2) Improper sample preparationAdd protease inhibitors and...
Read More5.3 Experimental Tips
Experimental Procedures1. Primary Antibody IncubationDilution ratio of primary antibody: For the first use, refer to the dilution ratio specified in the antibody instruction manual. If the antibody shows high sensitivity...
5.3 Experimental Tips
Experimental Procedures1. Primary Antibody IncubationDilution ratio of primary antibody: For the first use, refer to the dilution ratio specified in the antibody instruction manual. If the antibody shows high sensitivity...
Read More5.2.3 Sequential Incubation of Additional Targets on the Same Membrane
If the membrane needs to be incubated with antibodies for other targets subsequently, the target with relatively lower expression should be incubated first, followed by the one with higher expression....
5.2.3 Sequential Incubation of Additional Targets on the Same Membrane
If the membrane needs to be incubated with antibodies for other targets subsequently, the target with relatively lower expression should be incubated first, followed by the one with higher expression....
Read More5.2.2 Detection
Experimental Procedures1. Chemiluminescence Detection (1) Detection with Gel Imaging SystemFirst, place a flat piece of plastic wrap on the tray, then use forceps to lay the membrane flat on the...
5.2.2 Detection
Experimental Procedures1. Chemiluminescence Detection (1) Detection with Gel Imaging SystemFirst, place a flat piece of plastic wrap on the tray, then use forceps to lay the membrane flat on the...
Read More5.2.1 Antibody Incubation
Experimental Procedures1. TBST WashingWash the blocked membrane with 15 mL of TBST for 3 times, 5 minutes each time.2. Membrane CuttingIf the membrane needs to be cut into sections according...
5.2.1 Antibody Incubation
Experimental Procedures1. TBST WashingWash the blocked membrane with 15 mL of TBST for 3 times, 5 minutes each time.2. Membrane CuttingIf the membrane needs to be cut into sections according...
Read More5.1 Formulations of Reagents to Be Prepared for Antibody Incubation
Reagent Formulations1. Antibody DiluentFirst, prepare a 5% BSA (W/V, i.e., add 5 g of BSA to 100 mL of solution) using TBST. This serves as the antibody diluent for later...
5.1 Formulations of Reagents to Be Prepared for Antibody Incubation
Reagent Formulations1. Antibody DiluentFirst, prepare a 5% BSA (W/V, i.e., add 5 g of BSA to 100 mL of solution) using TBST. This serves as the antibody diluent for later...
Read More