7.3 NF-κB

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NF-κB1. NF-κB p65 Is an Important Transcription FactorNF-κB p65 is a crucial transcription factor. It can activate the regulation of genes involved in inflammation, cell proliferation, cell apoptosis, and other...

14 Dec 2025Bio Ant

7.3 NF-κB

NF-κB1. NF-κB p65 Is an Important Transcription FactorNF-κB p65 is a crucial transcription factor. It can activate the regulation of genes involved in inflammation, cell proliferation, cell apoptosis, and other...

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7.2 LC3B

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LC3B:1Autophagy is a catabolic process in which autophagolysosomes degrade most cytoplasmic contents.During autophagy, three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications. After synthesis, the carboxy-terminal of LC3...

14 Dec 2025Bio Ant

7.2 LC3B

LC3B:1Autophagy is a catabolic process in which autophagolysosomes degrade most cytoplasmic contents.During autophagy, three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications. After synthesis, the carboxy-terminal of LC3...

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7.1 Caspase 3

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Caspase 3 is generally regarded as the primary terminal cleavage enzyme in the process of apoptosis, a key executor of apoptosis, and also an important component of the cytotoxic T...

14 Dec 2025Bio Ant

7.1 Caspase 3

Caspase 3 is generally regarded as the primary terminal cleavage enzyme in the process of apoptosis, a key executor of apoptosis, and also an important component of the cytotoxic T...

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6.4 Others

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Troubleshooting 1. White Bands on Dark BackgroundPossible CausesExcessive sample loading volumeExcessively high concentrations of primary antibody and secondary antibodySolutionsIt is recommended to reduce the protein loading volume.Decrease the concentrations of...

14 Dec 2025Bio Ant

6.4 Others

Troubleshooting 1. White Bands on Dark BackgroundPossible CausesExcessive sample loading volumeExcessively high concentrations of primary antibody and secondary antibodySolutionsIt is recommended to reduce the protein loading volume.Decrease the concentrations of...

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6.3 Incorrect Band Size

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Troubleshooting 1. Inaccurate Indication by Pre-Stained Protein MarkerThe migration characteristics of pre-stained protein markers may change when electrophoresed under buffer conditions with different molecular weights, which can lead to certain...

14 Dec 2025Bio Ant

6.3 Incorrect Band Size

Troubleshooting 1. Inaccurate Indication by Pre-Stained Protein MarkerThe migration characteristics of pre-stained protein markers may change when electrophoresed under buffer conditions with different molecular weights, which can lead to certain...

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6.2 High Background & Multiple Bands

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Troubleshooting 1. Multiple Bands Appearing for the Target ProteinRule out post-translational modifications, protein isoforms, and cleavage products by consulting protein databases and literature.2. Sample-Related Causes(1) Sample DegradationAliquot the sample immediately...

14 Dec 2025Bio Ant

6.2 High Background & Multiple Bands

Troubleshooting 1. Multiple Bands Appearing for the Target ProteinRule out post-translational modifications, protein isoforms, and cleavage products by consulting protein databases and literature.2. Sample-Related Causes(1) Sample DegradationAliquot the sample immediately...

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