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THUNDER™ Total SMAD3 TR-FRET Cell Signaling Assay Kit

THUNDER™ Total SMAD3 TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-SMAD3T-5000 Brand: Bioauxilium
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$6,763 USD
$6,763 USD
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Product Details

Product Specification


Components


Eu-total-SMAD3
Acc-total-SMAD3
SMAD3 control lysate
5X Lysis Buffer 1
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail
Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Total SMAD3 (both phosphorylated and non-phosphorylated SMAD3) in cell lysates. This kit can be used to normalize data obtained with the Phospho-SMAD3 (S423/S425) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Picture

Stimulation of Phospho-SMAD3 (S423/S425) in A549 cells
Data show that treatment of A549 cells with TGFβ stimulates phosphorylation of SMAD3 at S423/S425, but does not affect the levels of total SMAD3.
Inhibition of Phospho-SMAD3 (S423/S425) in A549 cells
Data show that treatment of A549 cells with SB 431542 inhibits phosphorylation of SMAD3 at S423/S425 by TGFβ, but does not affect the levels of total SMAD3.
A549 control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values