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THUNDER™ Total BRD4 TR-FRET Cell Signaling Assay Kit

THUNDER™ Total BRD4 TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-BRD4-2500 Brand: Bioauxilium
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$5,441 USD
$5,441 USD
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Product Details

Product Specification


Components


Eu-Total BRD4
Acc-Total BRD4
BRD4 control lysate
5X Lysis Buffer 1
10X TR-FRET Detection buffer
10X Phosphatase Inhibitor Cocktail
Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Human Total BRD4 (both phosphorylated and non-phosphorylated) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Picture

Inhibition of Total BRD4 in MCF7 cells
Data show that treatment of MCF7 cells with the PROTAC compound dBET6 degrades total BRD4 in a concentration-dependent manner.
Inhibition of Total BRD4 in MCF7 cells
Data show that treatments of MCF7 cells with different PROTAC compounds degrade total BRD4. In contrast, BRD4 remains stable following treatment with an irrelevant compound.