THUNDER™ Phospho-SYK (Y525/Y526) + Total SYK TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-SYKPT Reactivity: Human, Mouse Conjugation: Brand: Bioauxilium

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$1,375 USD

Size: 500points

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Product Details

Product Specification

Antigen	SYK
Reactivity	Human, Mouse
Components	400 points for phospho; 100 points for total Eu-phospho-SYK Acc-phospho-SYK Eu-Total SYK Acc-Total SYK SYK control lysate 5X Lysis Buffer 3 10X TR-FRET Detection buffer 100X Phosphatase Inhibitor Cocktail
Product Description	Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-SYK (Y525/Y526) and Total SYK in cell lysates. This unique kit allows to simultaneously detect phosphorylated and total SYK proteins in separate wells within the same microplate. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

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Stimulation of Phospho-SYK (Y526/Y526) in Raji cells

Data show that treatment of Raji cells with pervanadate stimulates phosphorylation of SYK at Y525/Y526 but does not affect the levels of total SYK

Inhibition of Phospho-SYK (Y526/Y526) in U-937 cells

Data show that treatment of U-937 cells with BIIB-057 inhibits phosphorylation of SYK at Y525/Y526 by pervanadate, but does not affect the levels of total SYK

U-937 control lysate titration (QC Test) Phospho-SYK (Y525/Y526)

Data show a linear relationship between lysates pre-diluted at 1:4 or more and TR-FRET ratio values

U-937 control lysate titration (QC Test) Total SYK

Data show a linear relationship between lysate dilutions and TR-FRET ratio values

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THUNDER™ Phospho-SYK (Y525/Y526) + Total SYK TR-FRET Cell Signaling Assay Kit