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THUNDER™ Phospho-SMAD3 (S423/S425) TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-SMAD3 (S423/S425) TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-SMAD3P-5000 Brand: Bioauxilium
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$6,763 USD
$6,763 USD
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Product Details

Product Specification


Components


Eu-phospho-SMAD3
Acc-phospho-SMAD3
SMAD3 control lysate
5X Lysis Buffer 1
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail
Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-SMAD3 (S423/S425) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Picture

Stimulation of Phospho-SMAD3 (S423/S425) in A549 cells
Data show that treatment of A549 cells with TGFβ1 stimulates phosphorylation of SMAD3 at S423/S425.
Inhibition of Phospho-SMAD3 (S423/S425) in A549 cells
Data show that treatment of A549 cells with SB 431542 inhibits phosphorylation of SMAD3 at S423/S425 by TGFβ1.
Z'-factor determination in A549 cells
The Z’-factor value of 0.89 indicates that the assay is robust and suitable for HTS.
A549 control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.